Resveratrol protects human lens epithelial cells against H2O2- induced oxidative stress by increasing catalase, SOD-1, and HO-1 expression

文献类型： 外文期刊

第一作者： Zheng, Yi

作者： Zheng, Yi;Wang, Xiaoyuan;Liu, Lijuan;Liu, Ping;Liu, Yaohua;Ge, Jinying;Bu, Zhigao

作者机构：

期刊名称：MOLECULAR VISION （ 影响因子：2.367； 五年影响因子：3.037 ）

ISSN： 1090-0535

年卷期： 2010 年 16 卷 160-62 期

页码：

收录情况： SCI

摘要： Purpose: Oxidative damage induced by H2O2 treatment can irreversibly damage the lens epithelium, resulting in cell death and cataract. Whether the effects of oxidative stress could be attenuated in cultured human lens epithelial cells by incubation with resveratrol (RES) is still unknown. In the present study, we examined the function of resveratrol in protecting human lens epithelial B-3 (HLEB-3) cells against H2O2 induced cell death and cell apoptosis, its role in reducing H2O2 induced intracellular reactive oxygen species (ROS) accumulation, and investigated the mechanism by which resveratrol underlies the effect. Methods: HLEB-3 cells, a human lens epithelial cell line, were exposed to 100 mu M H2O2 with or without RES pretreatment at different concentrations for different time duration. Cell viabilities were monitored by 4-[3-[4-iodophenyl]-2-4(4-nitrophenyl)-2H-5-tetrazolio-1,3-benzene disulfonate] (WST-1) assay. The apoptosis rate and ROS generation were detected by flow cytometric analysis. Expression levels of superoxide dismutases-1 (SOD-1), catalase, and heme oxygenase-1 (HO-1) proteins were measured by western-blotting analysis. p38 and c-jun N terminal kinase (JNK) activation was also evaluated by western-blotting analysis. Results: Resveratrol clearly reduced H2O2 induced cell apoptosis and ROS accumulation; protected HLEB-3 cells from H2O2 induced oxidative damage, and increased the expression levels of SOD-1, catalase, and HO-1. Further studies showed that RES also inhibited H2O2 induced p38 and JNK phosphorylation. Conclusions: These findings suggested that RES protected HLEB-3 cells from H2O2 induced oxidative damage, presumably by inducing three antioxidative enzymes including catalase, SOD-1, and HO-1.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序