Screening and sequence analysis of the hemolysin gene of Fusobacterium necrophorum

文献类型： 外文期刊

第一作者： Miao, Liguang

作者： Miao, Liguang;Liu, Yanhuan;Li, Qingchao;Wang, Zhigang;Li, Haitao;Zhang, Guanglei

作者机构：

关键词： Fusobacterium necrophorum;Hemolysin;Genomic library

期刊名称：ANAEROBE （ 影响因子：3.331； 五年影响因子：3.539 ）

ISSN： 1075-9964

年卷期： 2010 年 16 卷 4 期

页码：

收录情况： SCI

摘要： Fusobacterium necrophorum is the main pathogen that causes numerous necrobacilloses. Hemolysin is one of the major virulence factors involved in fusobacterial infections. In order to investigate the genetic basis of hemolytic activity and the regulation mechanism of the hemolysin expression, a genomic library was constructed from F. necrophorum DNA by ligating DNA fragments generated by partial HindIII digestion with pUC18 vector. The screening of the genomic library with polymerase chain reaction, DNA sequencing and sequence assembly led to a 7.45 kb sequence which includes the putative hly gene and upstream sequence. Clustered putative genes encoding short chain acyl-CoA dehydrogenase (Scad) and electron transfer flavoprotein (Etf) alpha and beta subunits locate upstream of hly. A 535 bp non-coding sequence, possibly with some cis-regulatory elements involved in the regulation of the hemolysin expression in F. necrophorum, locates between etf-beta and hly. The nucleotide sequence of the hly gene indicates it encodes hemolysin. It is the first characterized hemolysin coding gene in E necrophorum. (C) 2010 Elsevier Ltd. All rights reserved.

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