Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV(FDDV13)

文献类型： 外文期刊

第一作者： Qi, Xu

作者： Qi, Xu;Su, Zhiqiang;Wang, Xuefeng;Wang, Shuai;Lin, Yuezhi;Jiang, Chenggang;Ma, Jian;Zhao, Liping;Lv, Xiaoling;Shen, Rongxian;Kong, Xiangang;Zhou, Jianhua;Wang, Xuefeng;Wang, Fenglong;Ma, Jian

作者机构：

关键词： EIAV;Vaccine;Gene;Characters;Comparison

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2010 年 41 卷 1 期

页码：

收录情况： SCI

摘要： Chinese equine infectious anemia virus (EIAV) attenuated vaccine is the first lentiviral vaccine with a successful application. In order to understand the correlation of viral genomic mutations with viral attenuation and with induced immunoprotective properties, we analyzed the proviral genome sequences of the EIAV-attenuated vaccine strain EIAV(FDDV13) (EIAV fetal donkey dermal cell-adapted vaccine) and its highly virulent parental strain EIAV(LN40). The sequences of these strains were compared with those of the major foreign EIAV strains. The results indicated a large genetic distance between the Chinese EIAV strain and the major EIAV strains in America and Japan. The Chinese strains belong to an independent phylogenetic branch. The divergence between the entire genome of the Chinese strains and that of other major EIAV strains is similar to 23%. The divergence rate in LTR is over 14%, whereas that in each open reading frame is over 20%. The gp90 exhibited a divergence of 35% in its nucleotide sequence and 40% in its amino acid sequence. The present study found that after long-term passage in vitro, EIAV(FDDV13) has accumulated many stable substitution mutations in each gene. These mutations at multiple sites in multiple genes of the vaccine strain, especially the conserved mutations, provide important references for further understanding the attenuation mechanism of Chinese EIAV-attenuated vaccine and the immunoprotection mechanism of lentiviral vaccines.

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