Expression of biologically active recombinant equine interferon-gamma in Escherichia coli

文献类型： 外文期刊

第一作者： Bai, Yu

作者： Bai, Yu;Tong, Tiegang;Liu, Guangliang;Chen, Weiye;Zhang, Weijun;Wang, Qun;Yang, Tao;Bu, Zhigao;Wu, Donglai;Bai, Yu

作者机构：

关键词： Interferon-gamma;Expression;Biological activity

期刊名称：COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES （ 影响因子：2.268； 五年影响因子：2.495 ）

ISSN： 0147-9571

年卷期： 2010 年 33 卷 4 期

页码：

收录情况： SCI

摘要： Interferon gamma (IFN-gamma) is a pleiotropic cytokine that is recognized as an important modulator of the immune response. To date, there is no report that prokaryocyte-derived recombinant equine IFN-gamma has antiviral activity. In this report, the gene coding equine IFN-gamma (EIFN-gamma) mature protein was cloned into pET-28a (+) and the recombinant EIFN-gamma was expressed in Escherichia coli (E. coli). The antiviral activity of expressed recombinant EIFN-gamma was evaluated by using a recombinant Vesicular Stornatits Virus expressing green fluorescence protein (rVSV-GFP) system in the equine fetal kidney-78 cell line (EFK-78). The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with EIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titer of antiviral activity was 1 x 10(3) AU/ml. These results demonstrated that the EIFN-gamma expressed in this study had good biological activity. Pure forms and sufficient quantities of biologically active IFN-gamma could facilitate the study of its activities in modulating immune responses both in vivo and in vitro. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.

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