Correlation between polymerase activity and pathogenicity in two duck H5N1 influenza viruses suggests that the polymerase contributes to pathogenicity

文献类型： 外文期刊

第一作者： Leung, Bo Wah

作者： Leung, Bo Wah;Brownlee, George G.;Chen, Hualan

作者机构：

关键词： H5N1;Influenza A/duck/Fujian/01/2002;Influenza A/duck/Guangxi/53/2002;RNA polymerase;PA subunit;PB2 subunit;Replication

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN： 0042-6822

年卷期： 2010 年 401 卷 1 期

页码：

收录情况： SCI

摘要： The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a high pathogenic, A/duck/Fujian/01/2002 (FJ) strain and a low pathogenic, A/duck/Guangxi/53/2002 (GX) strain. The polymerase activity of the FJ strain was significantly greater than the GX strain. Experiments with hybrid polymerase constructs - both in vitro and in ribonucleoprotein cell-based assays, suggested that the PA and to a lesser extent the PB2 subunits of the polymerase, were responsible for increased polymerase activity of the high pathogenic strain. However, promoter binding was inversely correlated with polymerase activity implying that excessive promoter binding inhibited polymerase activity by preventing promoter clearance. Overall, we suggest that the influenza polymerase is one of the determinants of pathogenicity of duck H5N1 viruses. (C) 2010 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Limited compatibility between the RNA polymerase components of influenza virus type A and B. Iwatsuki-Horimoto, Kiyoko,Muramoto, Yukiko,Kawaoka, Yoshihiro,Horimoto, Taisuke,Hatta, Yasuko,Hatta, Masato,Kawaoka, Yoshihiro,Chen, Hualan,Chen, Hualan. 2008

[2]Rifampicin-Resistance Mutations in the rpoB Gene in Bacillus velezensis CC09 have Pleiotropic Effects. Cai, Xun-Chao,Xi, Huan,Liang, Li,Liu, Jia-Dong,Liu, Chang-Hong,Xue, Ya-Rong,Yu, Xiang-Yang. 2017

[3]Recovery of infectious type Asia1 foot-and-mouth disease virus from suckling mice directly inoculated with an RNA polymerase I/II-driven unidirectional transcription plasmid. Lian, Kaiqi,Yang, Fan,Zhu, Zixiang,Cao, Weijun,Jin, Ye,Li, Dan,Zhang, Keshan,Guo, Jianhong,Zheng, Haixue,Liu, Xiangtao.

[4]H5N1 avian influenza in China. Chen HuaLan,Chen HuaLan. 2009

[5]A subunit vaccine candidate derived from a classic H5N1 avian influenza virus in China protects fowls and BALB/c mice from lethal challenge. Liu, Guangliang,Zhang, Fangfang,Shi, Jianzhong,Tian, Guobing,Chen, Hualan,Yu, Kangzheng,Meng, Qingwen. 2013

[6]Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus. Guo, Qian-Qian,Zhang, Zhan-Lu,Jiang, San-Jie,Ma, Jiang-Tao,Wu, Yan-Min,Zhang, Zhan-Lu,Xue, Wen-Tong,Ma, Jiang-Tao.

[7]Protective efficacy of an H1N1 cold-adapted live vaccine against the 2009 pandemic H1N1, seasonal H1N1, and H5N1 influenza viruses in mice. Shi, Jianzhong,Wen, Zhiyuan,Guo, Jing,Zhang, Ying,Deng, Guohua,Jiang, Yongping,Bu, Zhigao,Chen, Hualan,Shu, Yuelong,Wang, Dayan,Kawaoka, Yoshihiro.

[8]Avian influenza vaccines against H5N1 'bird flu'. Li, Chengjun,Bu, Zhigao,Chen, Hualan.

[9]Detection prevalence of H5N1 avian influenza virus among stray cats in eastern China. Zhao, Fu-Rong,Zhou, Dong-Hui,Zhang, Yong-Guang,Shao, Jun-Jun,Lin, Tong,Li, Yang-Fan,Wei, Ping,Chang, Hui-Yun,Zhao, Fu-Rong,Zhou, Dong-Hui,Zhang, Yong-Guang,Shao, Jun-Jun,Lin, Tong,Li, Yang-Fan,Wei, Ping,Chang, Hui-Yun,Wei, Ping.

[10]Changes in methylation of genomic DNA from chicken immune organs in response to H5N1 influenza virus infection. Zhang, Y. H.,Meng, J. L.,Gao, Y.,Zhang, J. Y.,Niu, S. L.,Sun, B. X.,Zhao, Z. H.,Yu, X. Z.,Li, Y. B.,Guan, Y. T.. 2016

[11]Generation and application of replication-competent Venus-expressing H5N1, H7N9, and H9N2 influenza A viruses. Wang, Guangwen,Zhang, Jie,Kong, Fandi,Li, Qibing,Wang, Jinliang,Ma, Shujie,Zhao, Yuhui,Liang, Libin,Li, Junping,Sun, Nan,Guan, Lizheng,Zhou, Yuan,Zhou, Chenchen,Huang, Shanyu,Bu, Zhigao,Jiang, Li,Chen, Hualan,Li, Chengjun. 2018

[12]Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses. Zhou, Zhen,Li, Xihan,Dong, Lei,Chen, Qun,Liu, Jialing,Hou, Dongxia,Zhang, Lin,Liu, Yuchen,Zhang, Yujing,Li, Jing,Wang, Jin,Chen, Xi,Zhang, Junfeng,Zen, Ke,Zhang, Chen-Yu,Liu, Jinxiong,Kong, Huihui,Zhang, Qianyi,Zhang, Guoquan,Qi, Xian,Wang, Hua. 2015

[13]Evaluation of Specific Pathogen-Free Ducks Infected with the Highly Pathogenic Avian Influenza Virus H5N1 Subtype Derived from Wild Birds. Guan, Xueting,Chai, Hongliang,Wang, Yulong,Hua, Yuping,Li, Yanbing,Liu, Jingli,Guan, Xueting. 2014

[14]Host cell interactome of PA protein of H5N1 influenza A virus in chicken cells. Wang, Qiao,Li, Qinghe,Liu, Ranran,Zheng, Maiqing,Wen, Jie,Zhao, Guiping.

[15]Lethal infection by a novel reassortant H5N1 avian influenza A virus in a zoo-housed tiger. He, Shang,Huang, Guoqing,Lu, Chengping,He, Shang,Lu, Chengping,He, Shang,Lu, Chengping,Shi, Jianzhong,Chen, Hualan,Qi, Xian.

[16]H5N1 influenza viruses: outbreaks and biological properties. Neumann, Gabriele,Kawaoka, Yoshihiro,Chen, Hualan,Gao, George F.,Shu, Yuelong,Kawaoka, Yoshihiro. 2010

[17]Protective Efficacy of an H5N1 DNA Vaccine Against Challenge with a Lethal H5N1 Virus in Quail. Li, Junping.

[18]Stable non-synonymous substitutions on NS gene (NS1 and NS2 proteins) of Qinghai Lake H5N1 influenza virus (Clade 2.2) after successive passages in Muscovy ducks. Song XiaoHui,Xiao HaiXia,Liu Di,Gao, George F.,Song XiaoHui,Xiao HaiXia,Liu Di,Gao, George F.,Song XiaoHui,Xiao HaiXia,Gao, George F.,Huang Yu,Gao, George F.. 2009

[19]Isolation and molecular characterization of an H5N1 swine influenza virus in China in 2015. Wu, Haibo,Yang, Fan,Liu, Fumin,Peng, Xiuming,Wu, Nanping,Lu, Rufeng,Xu, Lihua. 2018

[20]Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus. Qiao, Chuanling,Jiang, Yongping,Tian, Guobin,Wang, Xiurong,Li, Chengjun,Xin, Xiaoguang,Chen, Hualan,Yu, Kangzhen. 2009

作者其他论文 更多>>

微信小程序