BoPMEI1, a pollen-specific pectin methylesterase inhibitor, has an essential role in pollen tube growth

文献类型： 外文期刊

第一作者： Zhang, Guo Yu

作者： Zhang, Guo Yu;Feng, Jing;Wu, Jian;Wang, Xiao Wu

作者机构：

关键词： Broccoli (B. oleracea var. italica);Male sterility;Pectin methylesterase inhibitor;Pollen tube growth

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN： 0032-0935

年卷期： 2010 年 231 卷 6 期

页码：

收录情况： SCI

摘要： Pectin methylesterase (PME) is known to have important roles in pollen development and pollen tube growth. As pivotal regulatory factors in PME activity modulation, PME inhibitors (PMEIs) are thought to be key regulators of cell wall stability at the tip of the pollen tube. We report on the cloning and characterization of a novel B. oleracea PMEI gene, BoPMEI1. Heterologously expressed BoPMEI1 showed PMEI activity. RT-PCR studies of different tissues and promoter-GUS fusions confirmed that BoPMEI1 was specifically expressed in mature pollen grains and pollen tubes. Based on in vivo transient assays, we found that BoPMEI1 appears to be largely localized to the plasma membrane. Transgenic Arabidopsis plants expressing antisense BoPMEI1 under the control of the CaMV 35S promoter suppressed the expression of the orthologous gene At1g10770, which led to partial male sterility and decreased seed set by inhibition of pollen tube growth. Our study demonstrates the involvement of BoPMEI1 in pollen tube growth.

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