Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Wu, Ping

作者： Wu, Ping;Li, Mu-Wang;Guo, Xi-Jie;Jiang, Yun-Feng;Wang, Zi-Sheng;Guo, Xi-Jie

作者机构：

关键词： Bombyx mori;Cytoplasmic polyhedrosis virus;midgut;quantitative real-time PCR;rapid amplification of cDNA ends;suppression subtractive hybridization

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2010 年 17 卷 2 期

页码：

收录情况： SCI

摘要： Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the major viral pathogens for the silkworm. To date, the molecular mechanism of BmCPV invasion has been unclear. We cloned the full length complementary (c)DNA which encodes the ubiquitin-activating enzyme E1-domain containing protein1 (UbE1DC1) of Bombyx mori by using suppression subtractive hybridization (SSH) and rapid amplification of complementary (c)DNA ends (RACE). The full-length cDNA of UbE1DC1gene is 1 919 bp, consisting of a 100 bp 5' untranslated region, a 637 bp 3' untranslated region and an 1 182 bp open reading frame (ORF), encoding a 393 amino acid protein. The protein contained the THiF_MoeB_hesA_family domain, an adenosine triphosphate binding site, which belongs to the family of ubiquitin-activating enzyme E1. Reverse transcription - polymerase chain reaction analysis from the silkworm tissues, namely silk gland, hemocyte, fat body, gonad and midgut revealed that UbE1DC1 was expressed in all the five tissues. The real-time quantitative polymerase chain reaction analysis indicated that the relative expression of UbE1DC1 in the normal midgut was approximately 9.78-fold of that in the BmCPV-infected midgut. It is implicated that UbE1DC1 may play an important role in the interaction between the host and BmCPV invasion.

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