A Novel B-Cell Epitope of Avian Infectious Bronchitis Virus N Protein

文献类型： 外文期刊

第一作者： Yu, Dan

作者： Yu, Dan;Han, Zongxi;Xu, Jia;Shao, Yuhao;Li, Huixin;Kong, Xiangang;Liu, Shengwang

作者机构：

期刊名称：VIRAL IMMUNOLOGY （ 影响因子：2.257； 五年影响因子：1.882 ）

ISSN： 0882-8245

年卷期： 2010 年 23 卷 2 期

页码：

收录情况： SCI

摘要： This report describes the identification of a novel linear B-cell epitope at the N-terminus of the avian infectious bronchitis virus (IBV) nucleocapsid (N) protein. A monoclonal antibody designated 2D2 was prepared against the IBV N protein, and a series of 23 partially overlapping, tagged fragments of the IBV N gene were expressed. These peptides and five heterogeneous IBV strains were used to identify the epitope recognized by 2D2 using enzymelinked immunosorbent assay (ELISA) and Western blotting analysis. The linear motif 11 DSPAPIIKLGG21, located at the N-terminus of the N protein, was identified as the epitope bound by 2D2. ELISA and Western blotting also showed that this epitope was recognized by IBV-positive chicken serum. We propose that this motif is a linear B-cell epitope of N protein, and is the core sequence for reactivity, as demonstrated by binding of 2D2 to the truncated peptides and different IBV strains. Alignment and comparison of the 2D2-defined epitope with other coronavirus sequences showed that the epitope is conserved among avian coronaviruses. The identified epitope might be useful for clinical applications and as a tool for further study of the structure and function of the IBV N protein.

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