Characterization of a chromosomal segment showing xylanolytic activity from the symbiotic Sphingobacterium sp TN19

文献类型： 外文期刊

第一作者： Zhou, Junpei

作者： Zhou, Junpei;Meng, Kun;Yang, Peilong;Shi, Pengjun;Wang, Yaru;Luo, Huiying;Yao, Bin

作者机构：

关键词： Batocera horsfieldi;Sphingobacterium sp.;TN19;Gut;Xylan and xylose metabolism

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2010 年 26 卷 4 期

页码：

收录情况： SCI

摘要： A 37.5-kb chromosomal segment with 41.5% G+C content was cloned using genome walking method from Sphingobacterium sp. TN19, a symbiotic strain isolated from the gut of Batocera horsfieldi (Coleoptera, Cerambycidae) larvae. Twenty-three complete and two partial open reading frames (ORFs) were found in this region, and shared highest identities of 31.6-80.0% (11 of them < 60%) to known sequences from the sources shared the common feature-fluid with low dissolved oxygen levels. These ORFs were organized uniquely in chromosome compared to that from other bacteria, and putatively involved in xylan and xylose metabolism. To confirm their putative functions, one xylanase gene (xynB19) and one arabinosidase gene (gh43A19), were hetero-expressed in Escherichia coli BL21 (DE3), respectively, and both the recombinant enzymes showed significant enzymatic activities. This result indicates that xylan degradation is a complex process involving various enzymes, and genome walking is an efficient method to obtain multiple genes.

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