Cloning of a New Xylanase Gene from Streptomyces sp TN119 Using a Modified Thermal Asymmetric Interlaced-PCR Specific for GC-Rich Genes and Biochemical Characterization

文献类型： 外文期刊

第一作者： Zhou, Junpei

作者： Zhou, Junpei;Huang, Huoqing;Meng, Kun;Shi, Pengjun;Wang, Yaru;Luo, Huiying;Yang, Peilong;Bai, Yingguo;Yao, Bin

作者机构：

关键词： Xylanase;Batocera horsfieldi;Gut;Streptomyces sp.;TN119;Thermal asymmetric interlaced (TAIL)-PCR;High-GC genes

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2010 年 160 卷 5 期

页码：

收录情况： SCI

摘要： A bacterial strain, Streptomyces sp. TN119, was isolated from the gut of Batocera horsfieldi larvae and showed xylanolytic activity. A degenerate primer set was designed based on the base usage of G and C in Actinobacteria xylanase-coding sequences belonging to the glycosyl hydrolases family 10 (GH 10), and used to clone the partial xylanase gene from Streptomyces sp. TN119. A modified thermal asymmetric interlaced (TAIL)-PCR specific for high-GC genes, named GC TAIL-PCR, was developed to obtain the full-length xylanase gene (xynA119; 1089 bp). Rich in GC content (67.8%), xynA119 encodes a new GH 10 xylanase (XynA119), which shares highest identity (48.8%) with an endo-1,4-beta-xylanase from Cellulosimicrobium sp. HY-12. Recombinant XynA119 was expressed in Escherichia coli BL21 (DE3) and purified to electrophoretic homogeneity. The enzyme showed maximal activity at pH 6.5 and 60 A degrees C, was stable at pH 4.0 to 10.0 and 50 A degrees C, was resistant to most chemicals (except for Cu2+, Mn2+, Ag+, Hg2+ and SDS) and trypsin, and produced simple products. The specific activity, K (m), V (max), and k (cat) using oat-spelt xylan as substrate were 57.9 U mg(-1), 1.0 mg ml(-1), 74.8 mu mol min(-1) mg(-1), and 49.2 s(-1), respectively.

分类号：

  • 相关文献

[1]Molecular and biochemical characterization of a novel xylanase from the symbiotic Sphingobacterium sp TN19. Zhou, Junpei,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[2]Characterization of a chromosomal segment showing xylanolytic activity from the symbiotic Sphingobacterium sp TN19. Zhou, Junpei,Meng, Kun,Yang, Peilong,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yao, Bin. 2010

[3]Molecular cloning, expression, purification and characterization of a novel cellulase gene (Bh-EGaseI) in the beetle Batocera horsfieldi. Mei, Hui-Zhen,Xia, Ding-Guo,Zhao, Qiao-Ling,Zhang, Guo-Zheng,Qiu, Zhi-Yong,Qian, Ping,Mei, Hui-Zhen,Xia, Ding-Guo,Zhao, Qiao-Ling,Zhang, Guo-Zheng,Qiu, Zhi-Yong,Qian, Ping,Lu, Cheng,Xia, Ding-Guo.

[4]CDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi. Xia, Dingguo,Xiang, Zhonghuai,Lu, Cheng,Xia, Dingguo,Wei, Yadong,Zhang, Guozheng,Zhao, Qiaoling,Zhang, Yeshun.

[5]A new alpha-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for alpha-galactosidase activity of gastrointestinal bacteria. Zhou, Junpei,Shi, Pengjun,Huang, Huoqing,Cao, Yanan,Meng, Kun,Yang, Peilong,Zhang, Rui,Chen, Xiaoyan,Yao, Bin,Zhou, Junpei. 2010

[6]A new fungicide produced by a Streptomyces sp GAAS7310. Chen, GY,Lin, BR,Lin, YC,Xie, FC,Lu, W,Fong, WF. 2005

[7]Identification of highly-adhesive gut Lactobacillus strains in zebrafish (Danio rerio) by partial rpoB gene sequence analysis. Zhou, Zhigang,Wang, Wenwen,Liu, Wenshu,Zhang, Yuting,Yao, Bin,Gatlin, Delbert M., III,Ringo, Einar.

[8]A new degraded sesquiterpene from marine actinomycete Streptomyces sp 0616208. Xie, Xiu Chao,Mei, Wen Li,Zhao, You Xing,Hong, Kui,Dai, Hao Fu. 2006

[9]Paromomycin Derived from Streptomyces sp AG-P 1441 Induces Resistance against Two Major Pathogens of Chili Pepper. Sang, Mee Kyung,Park, Kyungseok,Kim, Chang-Jin,Park, Dong-Jin,Nam, Ki-Woong,Zhang, Kecheng.

[10]Benzamides and Quinazolines from a Mangrove Actinomycetes Streptomyces sp (No. 061316) and Their Inhibiting Caspase-3 Catalytic Activity in Vitro. Chen, Guodong,Gao, Hao,Tang, Jinshan,Huang, Yuanfan,Chen, Ying,Wang, Ying,Zhao, Huinan,Yao, Xinsheng,Lin, Haipeng,Xie, Qingyi,Hong, Kui,Gao, Hao,Li, Jia. 2011

[11]A novel low-temperature-active beta-glucosidase from symbiotic Serratia sp TN49 reveals four essential positions for substrate accommodation. Zhou, Junpei,Zhang, Rui,Shi, Pengjun,Huang, Huoqing,Meng, Kun,Yuan, Tiezheng,Yang, Peilong,Yao, Bin,Zhou, Junpei. 2011

[12]Identification of adherent microbiota in the stomach and intestine of emperor red snapper (Lutjanus sebae Cuvier) using 16S rDNA-DGGE. Zhou, Zhigang,Shi, Pengjun,He, Suxu,Liu, Yuchun,Huang, Guangxiang,Yao, Bin,Ringo, Einar,Ringo, Einar. 2009

[13]Dietary microbial phytase exerts mixed effects on the gut health of tilapia: a possible reason for the null effect on growth promotion. Hu, Jun,Zhang, Xuezhen,Hu, Jun,Ran, Chao,He, Suxu,Cao, Yanan,Yao, Bin,Zhou, Zhigang,Ye, Yuantu.

[14]Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus. Li Fenghui,Gao Fei,Tan Jie,Fan Chaojing,Sun Huiling,Yan Jingping,Chen Siqing,Li Fenghui,Fan Chaojing,Wang Xiaojun. 2016

[15]A new staurosporine analog from Actinomycetes Streptomyces sp (172614). Li, Xiao-Bin,Tang, Jin-Shan,Gao, Hao,Yao, Xin-Sheng,Ding, Rong,Yao, Xin-Sheng,Li, Jia,Hong, Kui. 2011

[16]Cytotoxic Constituents from Marine Actinomycete Streptomyces sp 124092. Xie Xiu-Chao,Mei Wen-Li,Zeng Yan-Bo,Lin Hai-Peng,Zhuang Ling,Dai Hao-Fu,Hong Kui,Xie Xiu-Chao. 2008

[17]Effects of dietary 60 g kg(-1) dried distiller's grains in least-cost practical diets on production and gut allochthonous bacterial composition of cage-cultured fish: comparison among fish species with different natural food habits. He, S.,Liu, Y.,Xu, L.,Zhou, Z.,Yao, B.,Wu, Z.,Wu, N.,Tao, Y.,Ringo, E..

[18]Dietary Additive Probiotics Modulation of the Intestinal Microbiota. Hu, Shenglan,Wang, Li,Jiang, Zongyong,Hu, Shenglan,Wang, Li,Jiang, Zongyong,Hu, Shenglan,Wang, Li,Jiang, Zongyong,Hu, Shenglan,Wang, Li,Jiang, Zongyong,Hu, Shenglan,Wang, Li,Jiang, Zongyong.

[19]Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers. Sun, Hong,Feng, Jie,Tang, Jiang-wu,Yao, Xiao-hong,Wu, Yi-fei,Wang, Xin.

[20]Gut immunity in Lepidopteran insects. Wu, Kai,Yang, Bing,Ling, Erjun,Huang, Wuren,Dobens, Leonard,Song, Hongsheng.

作者其他论文 更多>>

微信小程序