文献类型: 外文期刊
第一作者: Huang, Q. X.
作者: Huang, Q. X.;Cheng, X. Y.;Xu, R. M.;Xie, B. Y.
作者机构:
关键词: microsatellites;expressed sequence tags;plant-parasitic nematodes
期刊名称:HELMINTHOLOGIA ( 影响因子:1.184; 五年影响因子:0.96 )
ISSN: 0440-6605
年卷期: 2010 年 47 卷 1 期
页码:
收录情况: SCI
摘要: SSR markers have become the most popular resource for studying population genetic variation in eukaryotes. However, few studies with SSR markers have been carried out with phytoparasitic nematodes. In this study a primary survey on EST-SSRs was made utilizing bioinformatics methods to derive SSRs from expressed sequence tags (ESTs) of 16 species of PPN, which belong to 7 genera and 5 families. The results showed that trinucleotide repeats were the most abundant SSRs in coding ESTs, while tetranucleotide SSRs were predominant in non-coding ESTs and genome sequences. AG/CT, AAC/GTT and (AAAN)(n) motifs were predominant, and CG/GC, ACT/AGT motifs were scanty in the ESTs of most genera and species. SSRs were more abundant in non-coding ESTs than in coding ESTs. The distribution frequencies of SSR motifs in coding ESTs, non-coding ESTs and genomes are different. Our results will provide useful information for screening SSRs from each genus and species for further genetic study of phytoparastic nematodes.
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