Protein Cyclization Enhanced Thermostability and Exopeptidase-Resistance of Green Fluorescent Protein

文献类型： 外文期刊

第一作者： Zhao Zhonglin

作者： Zhao Zhonglin;Ma, Xin;Li, Liang;Zhang, Wei;Ping, Shuzhen;Lin, Min;Xu, Ming-Qun

作者机构：

关键词： Intein;protein cyclization;green fluorescent protein;purification

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN： 1017-7825

年卷期： 2010 年 20 卷 3 期

页码：

收录情况： SCI

摘要： A mutant of green fluorescent protein (GFPmut3*) from the jellyfish Aequorea victoria was cyclized in vitro and in vivo by the use of a naturally split intein from the dnaE gene of Synechocystis species PCC6803 (Ssp). Cyclization of GFPmut3* was confirmed by amino acid sequencing and resulted in an increased electrophoretic mobility compared with the linear GFPmut3*. The circular GFPmut3* was 5 C more thermostable than the linear form and significantly more resistant to proteolysis of exopeptidase. The circular GFPmut3* also displayed increased relative fluorescence intensity. In addition, chemical stability of GFPmut3* against GdnHCl revealed more stability of the circular form compared with the linear form.

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