In Vivo Monitor Oxidative Burst Induced by Cd2+ Stress for the Oilseed Rape (Brassica napus L.) Based on Electrochemical Microbiosensor

文献类型： 外文期刊

第一作者： Xu, Qiao

作者： Xu, Qiao;Wang, Zhan;Yang, Qin;Zhao, Yuan-Di;Wei, Fang;Chen, Hong

作者机构：

关键词： electrochemical microbiosensor;oilseed rape;oxidative burst;Cd2+ stress

期刊名称：PHYTOCHEMICAL ANALYSIS （ 影响因子：3.373； 五年影响因子：2.959 ）

ISSN： 0958-0344

年卷期： 2010 年 21 卷 2 期

页码：

收录情况： SCI

摘要： Introduction - Since the mechanism of Cd2+ stress for plants is not clear, an in vivo method to monitor Cd2+ stress for plants is necessary. However, oxidative burst (OB) is a signal messenger in the process of Cd2+ stress for plants. Objective - To establish an electrochemical method with poly-o-phenylenediamine and Pt microparticle modified Pt electrode (POPD-Pt-MP-Pt) as a microbiosensor for the in vivo detection of oxidative burst induced by Cd2+ stress in oilseed rape (Brassica napus L.). Methodology - The optimal fabrication of POPD-Pt-MP-Pt biosensor was achieved. Electrochemical signal was collected by amperometry. Results - After oilseed rape was exposed to 84.9 mm CdCl2 stress, three oxidative bursts were observed in oilseed rape by amperometry at 3.3 h, 8.4 h and 13.2 h, respectively. However, there was no obvious signal observed in the controlled assay. Conclusion - This contribution presents the in vivo monitoring of the OB process induced by Cd2+ stress in oilseed rape by POPD-Pt-MP-Pt microbiosensor in real-time. The novel electrochemical microbiosensor not only facilitates the real-time study in plant self-defence response to the adverse environment such as Cd2+ stress, but also provides an effective tool for probing the self-defence mechanism in plants. Copyright (C) 2009 John Wiley & Sons, Ltd.

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