Rapid detection and quantification of zearalenone-producing Fusarium species by targeting the zearalenone synthase gene PKS4

文献类型： 外文期刊

第一作者： Meng, Kun

作者： Meng, Kun;Wang, Yaru;Yang, Peilong;Luo, Huiying;Bai, Yingguo;Shi, Pengjun;Yuan, Tiezheng;Ma, Rui;Yao, Bin

作者机构：

关键词： Fusarium;Zearalenone;Real-time PCR;SYBR Green I

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2010 年 21 卷 2 期

页码：

收录情况： SCI

摘要： This work is the first report on developing a method to detect and quantify the zearalenone-producing Fusarium species in foodstuff by real-time PCR assay with SYBR Green I. Based on the polyketide synthase gene (PKS4) sequences of four zearalenone-producing Fusarium strains, a specific primer set was designed and used to detect and quantify zearalenone-producing Fusarium in foodstuff. The system developed under study required only 100 mg infected maize flour for test, had ability to detect down to 10 copies of the target gene per reaction, and produced reliable quantitative data over three orders of magnitude. Compared with the conventional methods, it is more rapid, specific and sensitive to detect potential zearalenone contamination in food or feed production. (C) 2009 Elsevier Ltd. All rights reserved.

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