Extremely Acidic beta-1,4-Glucanase, CelA4, from Thermoacidophilic Alicyclobacillus sp A4 with High Protease Resistance and Potential as a Pig Feed Additive

文献类型: 外文期刊

第一作者: Bai, Yingguo

作者: Bai, Yingguo;Wang, Jianshe;Shi, Pengjun;Luo, Huiying;Huang, Huoqing;Feng, Yukun;Yao, Bin;Zhang, Zhifang

作者机构:

关键词: Alicyclobacillus sp A4;cellulase;beta-1,4-glucanase;extremely acidic;high protease resistance

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN: 0021-8561

年卷期: 2010 年 58 卷 3 期

页码:

收录情况: SCI

摘要: An acidic endo-beta-1,4-glucanase, denoted CelA4 (similar to 48 kDa), was purified from thermoacidophilic Alicyclobacillus sp. A4. Two internal peptides of CelA4 showed strong sequence identity to the Alicyclobacillus acidocaldarius cellulase precursor and contained the conserved domain and catalytic region of glycoside hydrolase family 51 beta-1,4-glucanases, and the N-terminal and three other internal peptides had no close glucanase or cellulase relatives, suggesting that the enzyme might be novel. CelA4 had broad substrate specificity, exhibited maximum activity at 65 degrees C and pH 2.6, was stable over pH 1.8-7.6, and showed strong resistance to acidic and neutral proteases, notably pepsin. In comparison to the commercial endo-beta-1,3-1,4-glucanase, CelA4 was more stable, released more reducing Sugar from barley beta-glucan, and under simulated gastric conditions, decreased the viscosity of barley-.soybean feed to a greater extent. These properties make CelA4 a good candidate as a new commercial glucanase to improve the nutrient bioavailability of pig feed.

分类号:

  • 相关文献

[1]Purification, gene cloning and characterization of an acidic beta-1,4-glucanase from Phialophora sp G5 with potential applications in the brewing and feed industries. Zhao, Junqi,Shi, Pengjun,Yuan, Tiezheng,Huang, Huoqing,Li, Zhongyuan,Meng, Kun,Yang, Peilong,Yao, Bin. 2012

[2]Cloning, expression, and characterization of a thermostable beta-xylosidase from thermoacidophilic Alicyclobacillus sp A4. Zhang, Sanyan,Wang, Huimin,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Yao, Bin,Zhang, Sanyan,Xu, Bo. 2014

[3]A novel bifunctional GH51 exo-alpha-L-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp A4 with significant biomass-degrading capacity. Yang, Wenxia,Bai, Yingguo,Yang, Peilong,Luo, Huiying,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Yao, Bin. 2015

[4]Identification of an acidic alpha-amylase from Alicyclobacillus sp A4 and assessment of its application in the starch industry. Bai, Yingguo,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Yang, Peilong,Luo, Huiying,Luo, Chunliang,Feng, Yukun,Yao, Bin,Zhang, Wei. 2012

[5]A New alpha-Galactosidase from Thermoacidophilic Alicyclobacillus sp A4 with Wide Acceptor Specificity for Transglycosylation. Wang, Huimin,Ma, Rui,Shi, Pengjun,Xue, Xianli,Luo, Huiying,Huang, Huoqing,Bai, Yingguo,Yang, Peilong,Yao, Bin. 2014

[6]A novel xylanase, XynA4-2, from thermoacidophilic Alicyclobacillus sp A4 with potential applications in the brewing industry. Wang, Jianshe,Bai, Yingguo,Shi, Pengjun,Luo, Huiying,Huang, Huoqing,Yao, Bin,Wang, Jianshe,Yin, Jun. 2011

[7]Excellent waste biomass-degrading performance of Trichoderma asperellum T-1 during submerged fermentation. Wang, Qun,Shen, Qi,Zhao, Yuhua,Wang, Qun,Chen, Liang,Yu, Daobing,Lin, Hui. 2017

[8]Isolation and Identification of A Cellulolytic Bacterium from the Huguang Maar Lake. Cui, Lihong,Li, Jihua,Wei, Xiaoyi,Han, Donghui,Zhang, Yuliang. 2013

[9]Cellulase production by Trichoderma sp on apple pomace under solid state fermentation. Sun, Haiyan,Peng, Ming,Ge, Xiangyang,Hao, Zhikui. 2010

[10]Recyclable magnetic carboxymethyl chitosan/calcium alginate - cellulase bioconjugates for corn stalk hydrolysis. Jiang, Jianfang,Zhao, Jiaqi,He, Chunyang,Cui, Baodong,Xiong, Jun,Ao, Juan,Xiang, Guangyan,Jiang, Hao.

[11]Molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, Apriona germari. Wei, Ya Dong,Lee, Kwang Sik,Gui, Zhong Zheng,Yoon, Hyung Joo,Kim, Iksoo,Zhang, Guo Zheng,Guo, Xijie,Sohn, Hung Dae,Jin, Byung Rae.

[12]N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity. Wei, YD,Lee, KS,Gui, ZZ,Yoon, HJ,Kim, I,Je, YH,Lee, SM,Zhang, GZ,Guo, XJ,Sohn, HD,Jin, BR.

[13]Improvement of the catalytic performance of a Bispora antennata cellulase by replacing the N-terminal semi-barrel structure. Zheng, Fei,Wang, Xiaoyu,Xie, Xiangming,Zheng, Fei,Huang, Huoqing,Wang, Xiaoyu,Tu, Tao,Liu, Qiong,Meng, Kun,Wang, Yuan,Su, Xiaoyun,Luo, Huiying.

[14]Heterologous expression characteristics of trichoderma viride endoglucanase V in the Silkworm, Bombyx mori L.. Miao, Yun-gen,Li, Xing-hua,Wang, Mei-xian,Zhang, Peng,Hu, Jia-biao,Sun, Chun-guang,Zhou, Fang,Niu, Yan-shan,Malik, Firdose Ahmad,Bhaskar, Roy,Yang, Hua-jun,Miao, Yun-gen,Liu, Xin-ju.

[15]Fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives. Yu, Zhu,Zhao, Shufen,Cai, Yimin,Sun, Qizhong,Gao, Fengqin,Tao, Ya. 2012

[16]Revisiting overexpression of a heterologous beta-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities. Xue, Xianli,Ma, Rui,Luo, Huiying,Su, Xiaoyun,Yao, Bin,Wu, Yilan,Qin, Xing. 2016

[17]Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose. Chu, Yindi,Tu, Tao,Xue, Xianli,Wang, Xiaoyu,Gong, Li,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Penttinen, Leena,Rouvinen, Juha,Hakulinen, Nina,Yi, Zhuolin,Gong, Li. 2017

[18]The use of Agrobacterium-mediated insertional mutagenesis sequencing to identify novel genes of Humicola insolens involved in cellulase production. Fan, Chao,Xu, Xinxin,Guan, Weishi,Li, Jinyang,Liu, Bo,Zhang, Wei,Song, Liya,Shi, Pengjun. 2018

[19]Improving cellulase production in submerged fermentation by the expression of a Vitreoscilla hemoglobin in Trichoderma reesei. Lin, Jie,Zhang, Xiamei,Song, Bingran,Xue, Wei,Chen, Xiuzhen,Dong, Zhiyang,Lin, Jie,Zhang, Xiamei,Song, Bingran,Dong, Zhiyang,Su, Xiaoyun. 2017

[20]Identification and molecular characterization of a new beta-1,4-endoglucanase gene (Ha-eng-1a) in the cereal cyst nematode Heterodera avenae. Peng, Huan,Huang, Wenkun,Wang, Gaofeng,Peng, Deliang,Gao, Bingli,Moens, Maurice. 2012

作者其他论文 更多>>