Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA

文献类型： 外文期刊

第一作者： Liu, G. Q.

作者： Liu, G. Q.;Yun, T.;Zhang, Y. M.;Yun, T.;Ni, Z.;Yu, B.;Chen, L.;Huang, J. G.;Chen, J. P.

作者机构：

关键词： RNA viruses;Picornaviruses;Infectious cDNA;Duck hepatitis virus type 1

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2010 年 147 卷 2 期

页码：

收录情况： SCI

摘要： A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines. (C) 2009 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[2]Temperature-Dependent Survival of Turnip Crinkle Virus-Infected Arabidopsis Plants Relies on an RNA Silencing-Based Defense That Requires DCL2, AG02, and HEN1. Zhang, Xiuchun,Zhang, Xiaofeng,Singh, Jasleen,Qu, Feng,Zhang, Xiuchun,Zhang, Xiaofeng,Li, Dawei.

[3]Biochemical characterization of the small hydrophobic protein of avian metapneumovirus. Deng, Qiji,Demers, Andrew,Weng, Yuejin,Lu, Wuxun,Wang, Dan,Kaushik, Radhey S.,Li, Feng,Deng, Qiji,Demers, Andrew,Weng, Yuejin,Lu, Wuxun,Wang, Dan,Kaushik, Radhey S.,Li, Feng,Song, Minxun,Li, Feng,Yu, Qingzhong.

作者其他论文 更多>>

微信小程序