Cloning, expression and immunocompetence analysis of gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2

文献类型： 外文期刊

第一作者： Yang, Wei-Jun

作者： Yang, Wei-Jun;Yang, Zhi-Biao;Zheng, Sheng-Bo;Lan, Dao-Liang;Gu, Jiang-Ping;Xing, Rui;Yuan, Cong-Li;Cui, Li;Hua, Xiu-Guo;Cai, Xue-Hui;Yang, Wei-Jun;Hao, Yong-Qing

作者机构：

关键词： Streptococcus suis serotype 2;GDH;prokaryotic expression;Immunocompetence

期刊名称：2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010)

ISSN： 2151-7614

年卷期： 2010 年

页码：

收录情况： SCI

摘要： This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody. RESULTS: The fusion protein was efficiently expressed Western blotting analysis confirmed that the resulting protein was immunogenic. CONCLUSIONS: The GDH gene of S.suis 2 was successfully cloned and the high expressed, which facilitated the further studies on the development of immunological detection and vaccine design.

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