Identification of a novel S-RNase gene and S-genotypes of four pear (Pyrus pyrifolia) cultivars

文献类型： 外文期刊

第一作者： Zhang Lin

作者： Zhang Lin;Hu Jiao;Tan Xiaofeng;Long Hongxu;Yuan Deyi;Li Xiugen

作者机构：

关键词： Pyrus pyrifolia;gametophytic self-incompatibility (GSI);S-genotype;S-RNase;bioinformatics

期刊名称：2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010)

ISSN： 2151-7614

年卷期： 2010 年

页码：

收录情况： SCI

摘要： Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. Pyrus pyrifolia is a commercially important fruit tree that exhibits GSI. It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, an important local cultivar `Huobali' extensively used as parent in pear breeding programs, and its three progenies `Mantianhong', `Hongsucui' and `Meirensu' were used for S-genotyping analysis by PCR-based molecular method. Agarose gel electrophoresis showed that two fragments were produced in each of the three progenies, while three in the `Huobali'. After restriction digestion and sequencing analysis, the S-alleles in the four cultivars were determined. The allele of 345 bp from `Huobali' was determined as a novel S-RNase allele that was tentatively denominated as S-44-RNase. RT-PCR revealed that the S-44-RNase was expressed specifically in the styles, which is consistent with the expression pattern of S-RNases. Comparison of genomic and cDNA sequences revealed an intron of 148 bp for the S-44-RNase. At the amino acid level, it shared 65% to 93% similarity with other Maloideae S-RNases. This study will be helpful in pear production and breeding programs.

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