Gene cloning and expression of a new acidic family 7 endo-beta-1,3-1,4-glucanase from the acidophilic fungus Bispora sp MEY-1

文献类型： 外文期刊

第一作者： Luo, Huiying

作者： Luo, Huiying;Yang, Jun;Yang, Peilong;Huang, Huoqing;Shi, Pengjun;Bai, Yingguo;Wang, Yaru;Yao, Bin;Li, Jiang;Fan, Yunliu

作者机构：

关键词： Endo-beta-1,3-1,4-glucanase;Acidophilic fungus;Bispora sp MEY-1;Pichia pastoris

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2010 年 85 卷 4 期

页码：

收录情况： SCI

摘要： Most reported microbial beta-1,3-1,4-glucanases belong to the glycoside hydrolase family 16. Here, we report a new acidic family 7 endo-beta-1,3-1,4-glucanase (Bgl7A) from the acidophilic fungus Bispora sp. MEY-1. The cDNA of Bgl7A was isolated and over-expressed in Pichia pastoris, with a yield of about 1,000 U ml(-1) in a 3.7-l fermentor. The purified recombinant Bgl7A had three activity peaks at pH 1.5, 3.5, and 5.0 (maximum), respectively, and a temperature optimum at 60A degrees C. The enzyme was stable at pH 1.0-8.0 and highly resistant to both pepsin and trypsin. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan. The specific activity of Bgl7A against barley beta-glucan and lichenan (4,040 and 2,740 U mg(-1)) was higher than toward carboxymethyl cellulose sodium (395 U mg(-1)), which was different from other family 7 endo-beta-glucanases.

分类号：

  • 相关文献

[1]A thermophilic and acid stable family-10 xylanase from the acidophilic fungus Bispora sp MEY-1. Luo, Huiying,Yang, Jun,Wang, Hui,Yang, Yuhui,Huang, Huoqing,Shi, Pengjun,Yuan, Tiezheng,Yao, Bin,Li, Jiang,Fan, Yunliu.

[2]A novel highly acidic beta-mannanase from the acidophilic fungus Bispora sp MEY-1: gene cloning and overexpression in Pichia pastoris. Luo, Huiying,Wang, Yaru,Wang, Hui,Yang, Jun,Yang, Yuhui,Huang, Huoqing,Yang, Peilong,Bai, Yingguo,Shi, Pengjun,Yao, Bin,Fan, Yunliu.

[3]Cloning, expression and characterization of a novel acidic xylanase, XYL11B, from the acidophilic fungus Bispora sp MEY-1. Luo, Huiying,Wang, Yaru,Wang, Hui,Yang, Jun,Yang, Yuhui,Huang, Huoqing,Yao, Bin,Fan, Yunliu,Li, Jiang. 2009

[4]Cloning, expression and characterization of an acidic endo-polygalacturonase from Bispora sp. MEY-1 and its potential application in juice clarification. Yang, Jun,Luo, Huiying,Wang, Kun,Cheng, HaiPing,Bai, Yingguo,Yuan, Tiezheng,Yao, Bin,Li, Jiang,Fan, Yunliu. 2011

[5]Improvement of the thermostability and catalytic efficiency of a highly active beta-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge-charge interactions. You, Shuai,Tu, Tao,Wang, Yuan,Huang, Huoqing,Ma, Rui,Shi, Pengjun,Bai, Yingguo,Su, Xiaoyun,Luo, Huiying,Yao, Bin,Zhang, Lujia,Lin, Zhemin. 2016

[6]An alpha-galactosidase from an acidophilic Bispora sp MEY-1 strain acts synergistically with beta-mannanase. Wang, Hui,Luo, Huiying,Bai, Yingguo,Huang, Huoging,Shi, Pengjun,Yao, Bin,Li, Jiang,Fan, Yunliu. 2010

[7]An Acidophilic beta-Galactosidase from Bispora sp MEY-1 with High Lactose Hydrolytic Activity under Simulated Gastric Conditions. Wang, Hui,Luo, Huiying,Bai, Yingguo,Wang, Yaru,Yang, Peilong,Shi, Pengjun,Yao, Bin,Zhang, Wei,Fan, Yunliu. 2009

[8]Molecular cloning and expression of grass carp MyoD in yeast Pichia pastoris. Wang, Lixin,Bai, Junjie,Luo, Jianren,Chen, Hong,Ye, Xing,Jian, Qing,Lao, Haihua. 2007

[9]Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33. Mao, Ruoyu,Teng, Da,Wang, Xiumin,Zhang, Yong,Jiao, Jian,Cao, Xintao,Wang, Jianhua,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Zhang, Yong,Jiao, Jian,Cao, Xintao,Wang, Jianhua. 2015

[10]High expression of a heat-stable phytase in Pichia pastoris. Peng, RH,Xiong, AS,Li, X,Fan, HQ,Yao, QH,Guo, MJ,Zhang, SL. 2002

[11]Expression of tachyplesin gene in yeast Pichia pastoris. Zhang, CY,Zhao, J,Wu, CJ,Fan, YL. 1998

[12]Molecular identification and recombinant expression of red sea bream (Chrysophrys major) hepcidin gene. Chen, Song-Lin,Sui, Shao-Fei,Xu, Mei-Yu,Liu, Yang,Yang, Rong. 2005

[13]Cloning and overexpression of a Paenibacillus beta-glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. Yang, Peilong,Shi, Pengjun,Wang, Yaru,Bai, Yingguo,Meng, Kun,Luo, Huiying,Yuan, Tiezheng,Yao, Bin. 2007

[14]Molecular cloning and characterization of the novel acidic xylanase XYLD from Bispora sp MEY-1 that is homologous to family 30 glycosyl hydrolases. Luo, Huiying,Yang, Jun,Shi, Pengjun,Huang, Huoqing,Bai, Yingguo,Yao, Bin,Li, Jiang,Fan, Yunliu. 2010

[15]High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization. Gao, Zhaowei,Zhang, Yuhong,Li, Mu,Zhou, Liwei,Zhang, Wei,Gao, Zhaowei,Tang, Yunming,Li, Zhuofu,Huang, Huoqing,Yao, Bin. 2012

[16]Molecular cloning and characterization of a novel alpha-galactosidase gene from Penicillium sp F63 CGMCC 1669 and expression in Pichia pastoris. Mi, Shijun,Meng, Kun,Wang, Yaru,Bai, Yingguo,Yuan, Tiezheng,Luo, Huiying,Yao, Bin. 2007

[17]Cloning, Expression, and Characterization of a New Streptomyces sp S27 Xylanase for Which Xylobiose is the Main Hydrolysis Product. Li, Ning,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[18]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[19]Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dismutase and hCuZnSOD in Pichia pastoris. Qu He-zhi,Du shan-shan,Yang Shuo,Huang Lu,Zhang Lei,Xiao Song,Hao Dong-yun,Wang Xiao-ping,Hao Dong-yun. 2010

[20]Characterization of Interferon-alpha 1 of Yak. Chen, GuoHua,Jia, HuaiJie,Zhen, YaDong,Zeng, Shuang,Fang, YongXiang,He, XiaoBing,Jing, ZhiZhong,Cai, XuePeng. 2012

作者其他论文 更多>>

微信小程序