Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay

文献类型: 外文期刊

第一作者: Sui, Xinxia

作者: Sui, Xinxia;Wang, Linhai;Xia, Xianchun;He, Zhonghu;Sui, Xinxia;Wang, Zhenlin;He, Zhonghu

作者机构:

关键词: Functional marker;LMW-GS;multiplex PCR;Triticum aestivum L

期刊名称:CROP & PASTURE SCIENCE ( 影响因子:2.286; 五年影响因子:2.507 )

ISSN: 1836-5795

年卷期: 2010 年 61 卷 12 期

页码:

收录情况: SCI

摘要: Low-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of enduse products of common wheat. It is more efficient to discriminate LMW-GSalleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b,e,f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles.

分类号:

  • 相关文献

[1]Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat. Zhang, X. K.,Liu, L.,He, Z. H.,Sun, D. J.,He, X. Y.,Xu, Z. H.,Zhang, P. P.,Chen, F.,Xia, X. C.,Zhang, X. K.,He, Z. H.. 2008

[2]Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. H. Jin,J. Yan,R. J. Peña,X. C. Xia,A. Morgounov,L. M. Han,Y. Zhang,Z. H. He. 2011

[3]QTL mapping for quantities of protein fractions in bread wheat (Triticum aestivum L.). He, Zhonghu,Yan, Jun,Zhang, Yong,Tang, Jianwei,Zhang, Yelun,Yan, Jun,Xiao, Yonggui,Zhang, Yan,Xia, Xianchun,He, Zhonghu.

[4]Development of introgression lines with 18 alleles of glutenin subunits and evaluation of the effects of various alleles on quality related traits in wheat (Triticum aestivum L.). Li, Yulian,Zhou, Ronghua,Jia, Jizeng,Branlard, Gerard,Li, Yulian. 2010

[5]Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars. Ma, Wujun,Liu, Wan,Gao, Xuan,Wang, Ke,Wang, Shunli,Yan, Yueming,Zhang, Yanzhen,Zhang, Yong,He, Zhonghu,Zhang, Yong,He, Zhonghu. 2012

[6]Effects of Allelic Variation in Glutenin Subunits and Gliadins on Baking-quality in Near-isogenic Lines of Common Wheat cv. Longmai 19. Song, W. F.,Ren, Z. Y.,Zhang, Y. B.,Zhao, H. B.,Song, Q. J.,Zhang, C. L.,Xin, W. L.,Xiao, Z. M.,Song, W. F.,Ren, Z. Y.,Zhang, Y. B.,Li, J. L.,Guo, C. H.,Lv, X. B..

[7]Haplotype, molecular marker and phenotype effects associated with mineral nutrient and grain size traits of TaGS1 a in wheat. Guo, Ying,Sun, Jinjie,Zhang, Guizhi,Wang, Yingying,Kong, Fanmei,Zhao, Yan,Li, Sishen,Guo, Ying,Wang, Yingying. 2013

[8]Development and validation of a PCR-based functional marker system for the brown planthopper resistance gene Bph14 in rice. Zhou, Lei,Chen, Zhijun,Lang, Xuyong,Liu, Kai,Yang, Guocai,Hu, Gang,Li, Sanhe,You, Aiqing,Lang, Xuyong,Du, Bo,He, Guangcun. 2013

[9]Identification of blast resistance genes in 358 rice germplasms (Oryza sativa L.) using functional molecular markers. Yang, Yongyi,Ding, Hanfeng,Zhang, Hua,Xuan, Ning,Chen, Gao,Liu, Xu,Yao, Fangyi.

[10]Allelic variation of a soluble acid invertase gene (SAI-1) and development of a functional marker in sweet sorghum [Sorghum bicolor (L.) Moench]. Liu, Yang,Nie, Yuan-Dong,Han, Fen-Xia,Zhao, Xiang-Na,Dun, Bao-Qing,Lu, Ming,Li, Gui-Ying,Liu, Yang. 2014

[11]Functional Analysis and Marker Development of TaCRT-D Gene in Common Wheat (Triticum aestivum L.). Wang, Jiping,Li, Runzhi,Mao, Xinguo,Jing, Ruilian. 2017

[12]Cloning and haplotype analysis of TaSTE, which is associated with plant height in bread wheat (Triticum aestivum L.). Zhang, Wenping,Zhang, Lei,Qiao, Linyi,Lv, Wenyan,Wu, Jing,Zhao, Guangyao,Jing, Ruilian,Jia, Jizeng.

[13]A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice. Shao, G. N.,Tang, A.,Tang, S. Q.,Luo, J.,Jiao, G. A.,Wu, J. L.,Hu, P. S.. 2011

[14]Allelic Variation at the TaZds-A1 Locus on Wheat Chromosome 2A and Development of a Functional Marker in Common Wheat. Dong Chang-hai,Ma Zhi-ying,Dong Chang-hai,Xia Xian-chun,Zhang Li-ping,He Zhong-hu,He Zhong-hu,Zhang Li-ping. 2012

[15]Development and validation of a functional co-dominant SNP marker for the photoperiod thermo-sensitive genic male sterility pms3 (p/tms12-1) gene in rice. Qi, Yongbin,Wang, Linyou,Gui, Junmei,Zhang, Lixia,Liu, Qinglong,Wang, Jianjun. 2017

[16]Cloning and characterization of Tabas1-B1 gene associated with flag leaf chlorophyll content and thousand-grain weight and development of a gene-specific marker in wheat. Zhu, Xiao-Feng,Zhang, Hai-Ping,Hu, Ming-Jian,Wu, Zeng-Yun,Jiang, Hao,Cao, Jia-Jia,Xia, Xian-Chun,Ma, Chuan-Xi,Chang, Cheng,Xia, Xian-Chun.

[17]Elite Haplotypes of a Protein Kinase Gene TaSnRK2.3 Associated with Important Agronomic Traits in Common Wheat. Miao, Lili,Wang, Zhenhua,Mao, Xinguo,Wang, Jingyi,Liu, Zicheng,Zhang, Bin,Li, Weiyu,Chang, Xiaoping,Jing, Ruilian,Mao, Xinguo,Reynolds, Matthew. 2017

[18]Two members of TaRLK family confer powdery mildew resistance in common wheat. Tingting Chen,Jin Xiao,Jun Xu,Wentao Wan,Bi Qin,Aizhong Cao,Wei Chen,Liping Xing,Chen Du,Xiquan Gao,Shouzhong Zhang,Ruiqi Zhang,Wenbiao Shen,Haiyan Wang,Xiue Wang. 2016

[19]Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Zhang, X,Zhou, MP,Ren, LJ,Bai, GH,Ma, HX,Scholten, OE,Guo, PG,Lu, WZ. 2004

[20]Exploiting the diversity of Viviparous-1 gene associated with pre-harvest sprouting tolerance in European wheat varieties. Xia, L. Q.,Roeder, M. S.,Xia, L. Q.,He, Z. H.,Yang, Y.,Ganal, M. W.,Shewry, P. R.. 2008

作者其他论文 更多>>