Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay
文献类型: 外文期刊
第一作者: Sui, Xinxia
作者: Sui, Xinxia;Wang, Linhai;Xia, Xianchun;He, Zhonghu;Sui, Xinxia;Wang, Zhenlin;He, Zhonghu
作者机构:
关键词: Functional marker;LMW-GS;multiplex PCR;Triticum aestivum L
期刊名称:CROP & PASTURE SCIENCE ( 影响因子:2.286; 五年影响因子:2.507 )
ISSN: 1836-5795
年卷期: 2010 年 61 卷 12 期
页码:
收录情况: SCI
摘要: Low-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of enduse products of common wheat. It is more efficient to discriminate LMW-GSalleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b,e,f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles.
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