Functional Characterization of the Pheophytinase Gene, ZjPPH, From Zoysia japonica in Regulating Chlorophyll Degradation and Photosynthesis
文献类型： 外文期刊
第一作者： Teng, Ke
作者： Teng, Ke;Yue, Yuesen;Zhang, Hui;Li, Hui;Han, Chao;Fan, Xifeng;Wu, Juying;Xu, Lixin
作者机构：
关键词： pheophytinase; chlorophyll degradation; photosynthesis; chlorophyll a fluorescence; senescence; Zoysia japonica
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Pheophytinase (PPH), the phytol hydrolase, plays important roles in chlorophyll degradation. Nevertheless, little attention has been paid to the PPHs in warm-season grass species; neither its detailed function in photosynthesis has been systematically explored to date. In this study, we isolated ZjPPH from Zoysia japonica, an excellent warm-season turfgrass species. Quantitative real-time PCR analysis and promoter activity characterization revealed that the expression of ZjPPH could be induced by senescence, ABA, and dark induction. Subcellular localization observation proved that ZjPPH was localized in the chloroplasts. Overexpression of ZjPPH accelerated the chlorophyll degradation and rescued the stay-green phenotype of the Arabidopsis pph mutant. Moreover, ZjPPH promoted senescence with the accumulation of ABA and soluble sugar contents, as well as the increased transcriptional level of SAG12 and SAG14. Transmission electron microscopy investigation revealed that ZjPPH caused the decomposition of chloroplasts ultrastructure in stable transformed Arabidopsis. Furthermore, chlorophyll a fluorescence transient measurement analysis suggested that ZjPPH suppressed photosynthesis efficiency by mainly suppressing both photosystem II (PSII) and photosystem I (PSI). In conclusion, ZjPPH plays an important role in chlorophyll degradation and senescence. It could be a valuable target for genetic editing to cultivate new germplasms with stay-green performance and improved photosynthetic efficiency.
分类号：
- 相关文献
作者其他论文 更多>>
-
A ratiometric fluorescent probe for the determination of quinolone antibiotics in milk based on N and S co-doped carbon quantum dots
作者：Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Han, Jiajun;Gong, Wenwen
关键词：Carbon quantum dot; Ratiometric fluorescence probe; Moxifloxacin; Gatifloxacin; Ofloxacin; Milk
-
Metatranscriptomic insights into the mechanism of 'Multiple Qu' utilization in Jian-flavor Baijiu fermentation
作者：Xu, Shanshan;Hu, Weiqi;Zhou, Hao;Xu, Boyang;Wang, Ruijuan;Liu, Wuyang;Liu, Yong-xin;Li, Xingjiang;Liu, Yong-xin;Xu, Qinxiang;Zhang, Hui
关键词：Jian-flavor Baijiu; Multiple-qu fermentation; Single-qu fermentation; Metatranscriptomic analysis; Microbiota
-
Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846)
作者：Zeng, Lin;Wang, Yong-Hong;Ai, Chun-Xiang;Yu, Min-Hui;Liu, Bin;Liu, Bin;Zhang, Hui;Li, Fei
关键词：iron exposure; norfloxacin; NF-kappa B; HSP;
Larimichthys crocea -
No-tillage practice enhances soil total carbon content in a sandy Cyperus esculentus L. field
作者：Wang, Cong;Hu, Yuxiang;Wu, Hui;Wang, Zhirui;Cai, Jiangping;Wang, Zhengwen;Li, Hui;Wang, Cong;Hu, Yuxiang;Wu, Hui;Liu, Heyong;Jiang, Yong;Ren, Wei;Yang, Ning
关键词：No-tillage; Bacterial community composition; Bacterial function prediction; Aeolian sandy soil;
Cyperus esculentus L. -
Kynurenine, a derivative of tryptophan, inhibits progesterone biosynthesis in porcine granulosa luteal cells through Aryl hydrocarbon receptor-mediated downregulation of GATA-binding protein 4, 6, and CCAAT/enhancer-binding protein beta†
作者：Liao, Shiying;Deng, Yanfei;Liao, Shiying;Cheng, Jinhua;Zhao, Weimin;Dai, Chaohui;Fu, Yanfeng;Li, Bixia;Li, Hui;Li, Hui
关键词：kynurenine; progesterone; granulosa luteal cell; STAR; AHR
-
Comprehensive metabolite profiling reveals the dynamic changes of volatile and non-volatile metabolites in albino tea cultivar 'Ming guan' (MG) during white tea withering process☆
作者：Huang, Ting;Zhang, Yinggen;Wang, Xiuping;Zhang, Hui;Chen, Changsong;Chen, Quanbin;Zhong, Qiusheng
关键词：Albino tea cultivar; White tea; Metabolomics analysis; Dynamic changes; ROAVs
-
Genome-wide identification of the sugar beet (Beta vulgaris L.) DMP gene family and its potential role in abiotic stress
作者：Han, Pingan;Chang, Yue;Tang, Kuangang;Wang, Liang;Xiu, Zhijun;Yang, Jing;Shi, Haibo;Liang, Yahui;Sun, Ruifen;Su, Shaofeng;Zhang, Ziqiang;Fu, Zengjuan;Zhao, Shangmin;Yuanyuan, E.;Zheng, Wenzhe;Zhang, Hui;Zhang, Bizhou;Sun, Mengyuan;Wu, Xinrong;Li, Xiaodong
关键词：Sugar beet; DMP gene family; Bioinformatics; Abiotic stress; Gene expression
