DNA-chitosan nanoparticles improve DNA vaccine-elicited immunity against Newcastle disease virus through shuttling chicken interleukin-2 gene

文献类型： 外文期刊

第一作者： Zhang, Wenlong

作者： Zhang, Wenlong;Yin, Zhe;Liu, Nihong;Yang, Tao;Bu, Zhigao;Wu, Donglai;Zhang, Wenlong;Yin, Zhe;Wang, Junwei

作者机构：

期刊名称：JOURNAL OF MICROENCAPSULATION （ 影响因子：3.142； 五年影响因子：2.933 ）

ISSN： 0265-2048

年卷期： 2010 年 27 卷 8 期

页码：

收录情况： SCI

摘要： In this study, pCAGG-ChIL2 plasmid DNA containing the chicken interleukin-2 (ChIL-2) gene was used to prepare DNA-chitosan nanoparticles (CNPs). The CNPs prepared were spherical, with mean diameters between 100 and 200 nm, have a positive surface charge, and could protect DNA against DNase I degradation. The CNPs prepared were successfully used to transfect the Df-1 cell line with almost no cytotoxicity. CNPs prepared at an amino group to phosphate group ratio (N/P ratio) of 16 provided the highest transfection efficiency (1.1%) in medium with a pH of 6.5. When pCAGG-ChIL2 CNPs were administered to chickens simultaneously with a DNA vaccine against Newcastle disease virus (NDV), haemagglutination inhibition antibody titers and serum interferon-gamma (IFN-gamma) levels were significantly higher than in chickens immunised with the NDV DNA vaccine alone (p < 0.05). The results demonstrate that pCAGG-ChIL2 CNPs improve DNA vaccine-elicited immunity against NDV challenge.

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