SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit mu 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa)

文献类型: 外文期刊

第一作者: Qiao, Yongli

作者: Qiao, Yongli;Jiang, Wenzhu;Lee, JooHyun;Park, BongSoo;Choi, Min-Seon;Piao, Rihua;Woo, Mi-Ok;Paek, Nam-Chon;Seo, Hak Soo;Koh, Hee-Jong;Qiao, Yongli;Jiang, Wenzhu;Lee, JooHyun;Park, BongSoo;Choi, Min-Seon;Piao, Rihua;Woo, Mi-Ok;Paek, Nam-Chon;Seo, Hak Soo;Koh, Hee-Jong;Roh, Jae-Hwan;Han, Longzhi

作者机构:

关键词: clathrin-associated adaptor protein;early senescence;hypersensitive response;lesion mimic mutant;rice (Orzya sativa);spotted leaf 28

期刊名称:NEW PHYTOLOGIST ( 影响因子:10.151; 五年影响因子:10.475 )

ISSN: 0028-646X

年卷期: 2010 年 185 卷 1 期

页码:

收录情况: SCI

摘要: P> To expand our understanding of cell death in plant defense responses, we isolated a novel rice (Oryza sativa) spotted leaf mutant (spl28) that displays a lesion mimic phenotype in the absence of pathogen attack through treatment of Hwacheongbyeo (an elite Korean japonica cultivar) with N-methyl-N-nitrosourea (MNU). Early stage development of the spl28 mutant was normal. However, after flowering, spl28 mutants exhibited a significant decrease in chlorophyll content, soluble protein content, and photosystem II efficiency, and high concentrations of reactive oxygen species (ROS), phytoalexin, callose, and autofluorescent phenolic compounds that localized in or around the lesions. The spl28 mutant also exhibited significantly enhanced resistance to rice blast and bacterial blight. Using a map-based cloning approach, we determined that SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit mu 1 (AP1M1), which is involved in the post-Golgi trafficking pathway. A green fluorescent protein (GFP) fusion protein of SPL28 (SPL28::GFP) localized to the Golgi apparatus, and expression of SPL28 complemented the membrane trafficking defect of apm1-1 Delta yeast mutants. SPL28 was ubiquitously expressed and contained a highly conserved adaptor complex medium subunit (ACMS) family domain. SPL28 appears to be involved in the regulation of vesicular trafficking, and SPL28 dysfunction causes the formation of hypersensitive response (HR)-like lesions, leading to the initiation of leaf senescence.

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