Recovery of infectious foot-and-mouth disease virus from full-length genomic cDNA clones using an RNA polymerase I system

文献类型： 外文期刊

第一作者： Chang, Yanyan

作者： Chang, Yanyan;Zheng, Haixue;Shang, Youjun;Jin, Ye;Wang, Guangxiang;Shen, Xiaoyan;Liu, Xiangtao

作者机构：

关键词： foot-and-mouth disease virus;RNA polI-based system;infectious cDNA clone

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 1672-9145

年卷期： 2009 年 41 卷 12 期

页码：

收录情况： SCI

摘要： The prototypic foot-and-mouth disease virus (FMDV) was shown more than a century ago to be the first filterable agent capable of causing FMD, and it has served as an important model for studying basic principles of Aphthovirus molecular biology. However, the complex structure and antigenic diversity of FMDV have posed a major obstacle to the attempts at manipulating the infectious virus by reverse genetic techniques. Here, we report the recovery of infectious FMDV from cDNAs based on an efficient in vivo RNA polymerase (pol) transcription system. Intracellular transcription of the full-length viral genome from pol-based vectors resulted in efficient formation of infectious virus displaying a genetic marker. Compared with wild-type virus, an abundance of genomic mRNA and elevated expression levels of viral antigens were indicative of the hyperfunction throughout the life-cycle of this cDNA-derived virus at transcription, replication, and translation levels. The technology described here could be an extremely valuable molecular biology tool for studying FMDV complex infectious characteristics. It is an operating platform for studying FMDV functional genomics, molecular mechanism of pathogenicity and variation, and lays a solid foundation for the development of viral chimeras toward the prospect of a genetically engineered vaccine.

分类号：

  • 相关文献

[1]Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning. Tuo, Decai,Shen, Wentao,Yan, Pu,Li, Xiaoying,Zhou, Peng. 2015

[2]Pig BVDV-2 non-structural protein (N-pro) links to cellular antiviral response in vitro. Tao, Jie,Liao, Jinhu,Wang, Jianye,Zhang, Xinjun,Zhang, Qian,Zhu, Liqian,Zhu, Guoqiang,Tao, Jie,Liao, Jinhu,Wang, Jianye,Zhang, Xinjun,Zhang, Qian,Zhu, Liqian,Zhu, Guoqiang,Tao, Jie,Liu, Huili,Zhang, Weiping.

[3]CpG DNA enhances the immune responses elicited by the DNA vaccine against foot-and-mouth disease virus in guinea pigs. Li, GJ,Li, YJ,Yan, WY,Xu, QX,Wu, YQ,Xie, Y,You, YJ,Zheng, ZX. 2001

[4]Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine. Du, Yijun,Li, Yufeng,Jiang, Wenming,Wang, Xinglong,Tang, Bo,Cao, Jun,Wang, Xianwei,Jian, Ping,He, Hairong,Qi, Jing. 2008

[5]The codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. Zhou, Jian-hua,Zhang, Jie,Chen, Hao-tai,Ma, Li-na,Ding, Yao-zhong,Liu, Yong-sheng,Pejsak, Zygmunt. 2011

[6]Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin beta 6-1 Subunit. Zhang, Yan,Sun, Yingjun,Yang, Fan,Guo, Jianhong,He, Jijun,Wu, Qiong,Cao, Weijun,Lv, Lv,Zheng, Haixue,Zhang, Zhidong. 2013

[7]Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1. Jiang, Tao,Liang, Zhong,Ren, Weiwei,Chen, Juan,Zhi, Xiaoying,Qi, Guangyu,Yang, Yamin,Liu, Zaixing,Liu, Xiangtao,Cai, Xuepeng,Ren, Weiwei,Zhi, Xiaoying,Qi, Guangyu. 2011

[8]An increased replication fidelity mutant of foot-and-mouth disease virus retains fitness in vitro and virulence in vivo. Zeng, Jianxiong,Wang, Haiwei,Xie, Xiaochun,Yang, Decheng,Zhou, Guohui,Yu, Li.

[9]Induction of systemic IFITM3 expression does not effectively control foot-and-mouth disease viral infection in transgenic pigs. Zhang, Huawei,Qian, Ping,Xu, Jinfang,Yang, Xi,Zhou, Rui,Chen, Huanchun,Li, Xiangmin,Zhang, Huawei,Qian, Ping,Xu, Jinfang,Yang, Xi,Zhou, Rui,Chen, Huanchun,Li, Xiangmin,Zheng, Haixue.

[10]Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast. Sun, M,Qian, KX,Su, N,Chang, HY,Liu, JX,Chen, GF.

[11]Construction and characterization of 3A-epitope-tagged foot-and-mouth disease virus. Ma, Xueqing,Li, Pinghua,Sun, Pu,Bai, Xingwen,Bao, Huifang,Lu, Zengjun,Fu, Yuanfang,Cao, Yimei,Li, Dong,Chen, Yingli,Liu, Zaixin,Qiao, Zilin.

[12]Foot-and-mouth disease virus induces lysosomal degradation of host protein kinase PKR by 3C proteinase to facilitate virus replication. Li, Chuntian,Zhu, Zixiang,Du, Xiaoli,Cao, Weijun,Yang, Fan,Zhang, Xiangle,Feng, Huanhuan,Li, Dan,Zhang, Keshan,Liu, Xiangtao,Zheng, Haixue.

[13]Purification of foot-and-mouth disease virus by heparin as ligand for certain strains. Du, Ping,Sun, Shiqi,Dong, Jinjie,Zhi, Xiaoying,Chang, Yanyan,Teng, Zhidong,Guo, Huichen,Liu, Zaixin.

[14]Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine. Zheng, Haixue,Lian, Kaiqi,Yang, Fan,Jin, Ye,Zhu, Zixiang,Guo, Jianhong,Cao, Weijun,Liu, Huanan,He, Jijun,Zhang, Keshan,Li, Dan,Liu, Xiangtao.

[15]Characteristics of codon usage bias in two regions downstream of the initiation codons of foot-and-mouth disease virus. Zhou, Jian-hua,Zhang, Jie,Ding, Yao-zhong,Chen, Hao-tai,Ma, Li-na,Liu, Yong-sheng.

[16]Effect of the nucleotides surrounding the start codon on the translation of foot-and-mouth disease virus RNA. Ma, X. X.,Feng, Y. P.,Ma, Z. R.,Gu, Y. X.,Ma, X. X.,Zhou, J. H..

[17]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[18]Identification of a conformational neutralizing epitope on the VP1 protein of type A foot-and-mouth disease virus. Liu, Wenming,Yang, Baolin,Wang, Mingxia,Wang, Haiwei,Yang, Decheng,Zhou, Guohui,Yu, Li,Ma, Wenge.

[19]Chemiluminescence Immunoassay for the Detection of Antibodies against the 2C and 3ABC Nonstructural Proteins Induced by Infecting Pigs with Foot-and-Mouth Disease Virus. Liu, Zezhong,Shao, Junjun,Zhao, Furong,Zhou, Guangqing,Gao, Shandian,Liu, Wei,Lv, Jianliang,Li, Yangfan,Chang, Huiyun,Zhang, Yongguang,Zhang, Yongguang,Li, Xiumei.

[20]Protection of a novel epitope-RNA VLP double-effective VLP vaccine for foot-and-mouth disease. Dong, Yan-mei,Chen, Liang,Dong, Yan-mei,Cai, Jian-chun,Chen, Hao-tai.

作者其他论文 更多>>

微信小程序