Naturally occurring mutations at residues 253 and 284 in VP2 contribute to the cell tropism and virulence of very virulent infectious bursal disease virus

文献类型： 外文期刊

第一作者： Qi, Xiaole

作者： Qi, Xiaole;Gao, Honglei;Gao, Yulong;Qin, Liting;Wang, Yongqiang;Gao, Li;Wang, Xiaomei;Qi, Xiaole

作者机构：

关键词： Infectious bursal disease virus (IBDV);VP2;Cell tropism;Virulence

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

ISSN： 0166-3542

年卷期： 2009 年 84 卷 3 期

页码：

收录情况： SCI

摘要： Infectious bursal disease virus (IBDV) is responsible for the highly contagious infectious bursal disease in chickens. Previously, by blind passage, a vvIBDV Gx strain was attenuated to the Gt strain, and a strain CEF-9 with intermediate characters was obtained during attenuation. Since CEF-9 exhibited only two interesting amino acid mutations (Q253H and A284T) on loops P(DE) and P(FG) at the tip of VP2 spikes, we hypothesized that, either function separately or in combination, they define the cell tropism and virulence of vvIBDV. To test this hypothesis, Q253H and A284T were introduced individually or in combination into VP2 of the Gx or Gt strain to obtain six modified clones. Using reverse genetics, combined mutations of Q253H and A284T could adapt vvIBDV to non-permissive CEF cells (rGx-F9VP2) but any single mutation could not. In vivo, rGx-F9VP2 did not cause mortality while the Gx strain induced 66.7% mortality. Dual evidence from natural and rescued strains identified that the cell tropism of vvIBDV to CEF cells was determined by the combined VP2 mutations Q253H and A284T, but not by single mutation. The two residues were mainly responsible for the virulence of vvIBDV. These findings may be helpful in the design of new tailored IBDV vaccines. (C) 2009 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Analysis of the function of D279N mutation of VP2 of infectious bursal disease virus. Qi Xiao-le,Lu Zhen,Wang Nian,Chen Yu-ming,Zhang Li-zhou,Gao Li,Li Kai,Ren Xian-gang,Wang Yong-qiang,Gao Hong-lei,Gao Yu-long,Wang Xiao-mei,Eterradossi, Nicolas,Wang Xiao-mei. 2015

[2]A single mutation in the P-BC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus. Qi, Xiaole,Gao, Xiang,Lu, Zhen,Zhang, Lizhou,Wang, Yongqiang,Gao, Li,Gao, Yulong,Li, Kai,Gao, Honglei,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Wang, Xiaomei,Wang, Xiaomei. 2016

[3]Effects of amino acid substitutions in the VP2 B-C loop on antigenicity and pathogenicity of serotype Asia1 foot-and-mouth disease virus. Xue, Mei,Wang, Haiwei,Li, Wan,Zhou, Guohui,Tu, Yabin,Yu, Li. 2012

[4]Antigenic structure analysis of VP3 of infectious bursal disease virus. Deng, Xiaoyun,Gao, Yulong,Gao, Honglei,Qi, Xiaole,Cheng, Yu,Wang, Xiaoyan,Wang, Xiaomei. 2007

[5]Overexpression of microRNA gga-miR-21 in chicken fibroblasts suppresses replication of infectious bursal disease virus through inhibiting VP1 translation. Ouyang, Wei,Pan, Qun-xing,Wang, Xiao-li,Xia, Xing-xia,Bi, Zhen-wei,Wang, Yong-qiang,Wang, Xiao-mei.

[6]Naturally occurring reassortant infectious bursal disease virus in northern China. Lu, Zhen,Zhang, Lizhou,Wang, Nian,Chen, Yuming,Gao, Li,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Li, Kai,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[7]A reassortment vaccine candidate as the improved formulation to induce protection against very virulent infectious bursal disease virus. Qi, Xiaole,Chen, Yuming,Ren, Xiangang,Zhang, Lizhou,Gao, Li,Wang, Nian,Qin, Liting,Wang, Yongqiang,Gao, Yulong,Wang, Xiaomei. 2014

[8]Effective inhibition of infectious bursal disease virus replication in vitro by DNA vector-based RNA interference. Gao, Yulong,Liu, Wei,Gao, Honglei,Qi, Xiaole,Lin, Huan,Wang, Xiaomei,Shen, Rongxian,Gao, Yulong,Wang, Xiaomei. 2008

[9]gga-miR-9*inhibits IFN production in antiviral innate immunity by targeting interferon regulatory factor 2 to promote IBDV replication. Ouyang, Wei,Liu, Hua-jie,Zhang, Hai-bin,Ouyang, Wei,Wang, Yong-shan,Du, Xi-ning,Liu, Hua-jie,Ouyang, Wei,Wang, Yong-shan.

[10]Function of VP2 Protein in the Stability of the Secondary Structure of Virus-like Particles of Genogroup II Norovirus at Different pH Levels: Function of VP2 Protein in the Stability of NoV VLPs. Yao Lin,Li Fengling,Wang Lianzhu,Zhai Yuxiu,Jiang Yanhua. 2014

[11]The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection. Zhang, Lizhou,Li, Hui,Chen, Yuming,Gao, Xiang,Lu, Zhen,Gao, Li,Wang, Yongqiang,Gao, Yulong,Gao, Honglei,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Pan, Qing,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[12]Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens. Liu, Linlin,Zhang, Wang,Song, Yuxin,Wang, Wenqian,Zhang, Yuan,Wang, Tingting,Li, Kai,Pan, Qing,Qi, Xiaole,Gao, Yulong,Gao, Li,Liu, Changjun,Zhang, Yanping,Wang, Yongqiang,Wang, Xiaomei,Cui, Hongyu,Zhang, Wang,Song, Yuxin,Zhang, Yuan,He, Gaoming. 2018

[13]Identification of a serotype-independent linear epitope of foot-and-mouth disease virus. Yang, Baolin,Wang, Mingxia,Liu, Wenming,Xu, Zhiqiang,Wang, Haiwei,Yang, Decheng,Zhou, Guohui,Yu, Li,Ma, Wenge. 2017

[14]Identification of the interaction and interaction domains of chicken anemia virus VP2 and VP3 proteins. Sun, Fenfen,Pan, Wei,Gao, Honglei,Qi, Xiaole,Qin, Liting,Wang, Yongqiang,Gao, Yulong,Wang, Xiaomei,Sun, Fenfen,Pan, Wei. 2018

[15]Typing of Canine Parvovirus Strains Circulating in North-East China. Zhao, H.,Wang, J.,Cheng, Y.,Lin, P.,Zhu, H.,Yi, L.,Zhang, S.,Guo, L.,Cheng, S.,Jiang, Y.,Han, G..

[16]Mapping of epitopes of VP2 protein of chicken anemia virus using monoclonal antibodies. Wang, Xiaoyan,Gao, Honglei,Gao, Yulong,Fu, Chaoyang,Wang, Zhi,Lu, Guili,Cheng, Yu,Wang, Xiaomei. 2007

[17]Molecular epidemiology of Aleutian mink disease virus in China. Wang, Zhenjun,Wu, Wei,Hu, Bo,Zhang, Hailing,Bai, Xue,Zhao, Jianjun,Zhang, Lei,Yan, Xijun.

[18]Fine Mapping of a Foot-and-mouth Disease Virus Epitope Recognized by Serotype-Independent Monoclonal Antibody 4B2. Yu, Yongzhong,Wang, Haiwei,Zhao, Lei,Zhang, Chunyuan,Jiang, Zhigang,Yu, Li.

[19]Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus. Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Tan, Yeping.

[20]Multiple adaptive amino acid substitutions increase the virulence of a wild waterfowl-origin reassortant H5N8 avian influenza virus in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Cheng, Kaihui,Xia, Xianzhu,Gao, Yuwei. 2018

作者其他论文 更多>>

微信小程序