Protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing IBV-S1 and chicken IFN gamma
文献类型: 外文期刊
第一作者: Wang, Yun-Feng
作者: Wang, Yun-Feng;Sun, Yong-Ke;Tian, Zhan-Cheng;Shi, Xing-Ming;Tong, Guang-Zhi;Liu, Sheng-Wang;Zhi, Hai-Dong;Kong, Xian-Gang;Wang, Mei
作者机构:
关键词: Infectious bronchitis virus;S1 gene;Recombinant fowlpox virus;Chicken type II interferon
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN: 0264-410X
年卷期: 2009 年 27 卷 50 期
页码:
收录情况: SCI
摘要: A fowlpox virus expressing the chicken infectious bronchitis virus (IBV) S1 gene of the LX4 strain (rFPV-IBVS1) and a fowlpox virus co-expressing the S1 gene and the chicken type II interferongene (rFPV-IBVS1-ChIFN gamma) were constructed. These viruses were assessed for their immunological efficacy on specific-pathogen-free (SPF) chickens challenged with a virulent IBV. Although the antibody levels in the rFPV-IBVS1-ChIFN-gamma-vaccinated group were lower than those in the attenuated live IB vaccine H120 group and the rFPV-IBVS1 group, the rFPV-IBVS1 -ChIFN gamma provided the strongest protection against an IBV LX4 virus challenge (15 out of 16 chickens immunized with rFPV-IBVS1-ChIFN gamma were protected), followed by the attenuated live IB vaccine (13/16 protected) and the rFPV-IBVS1 (12/16 protected). Compared to those of the rFPV-IBVS1 and the attenuated live IB vaccine groups, chickens in the rFPV-IBVS1-ChIFN gamma group eliminated virus more quickly and decreased the presence of viral antigen more significantly in renal tissue. Examination of affected tissues revealed abnormalities in the liver, spleen, kidney, lung and trachea of chickens vaccinated with the attenuated live IB vaccine and the rFPV-IBVS1 vaccine. In rFPV-IBVS1 -ChIFN gamma-vaccinated chickens, pathological changes were also observed in those organs, but were milder and lasted shorter. The lesions in the mock control group were the most severe and lasted for at least 20 days. This study demonstrated that chicken type II interferon increased the immunoprotective efficacy of rFPV-IBVS1-ChIFN gamma and normal weight gain in vaccinated chickens although it inhibited serum antibody production. (C) 2009 Elsevier Ltd. All rights reserved.
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