Two Novel Duck Antibacterial Peptides, Avian beta-Defensins 9 and 10, with Antimicrobial Activity (Retracted article. See vol. 23, pg. 1046, 2013)
文献类型: 外文期刊
第一作者: Liao, Wenyan
作者: Liao, Wenyan;Wang, Ruiqin;Han, Zongxi;Liu, Shengwang;Ma Deying;Liao, Wenyan;Wang, Ruiqin
作者机构:
关键词: Antimicrobial peptides;duck;avian beta-defensins;antimicrobial activity
期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )
ISSN: 1017-7825
年卷期: 2009 年 19 卷 11 期
页码:
收录情况: SCI
摘要: Two novel avian beta-defensins (AvBDs) isolated from duck liver were characterized and their homologies with other AvBDs were analyzed. They were shown to be duck AvBD9 and AvBD10. The mRNA expression of the two genes was analyzed in 17 different tissues from 1-28-day-old ducks. AvBD9 was differentially expressed in the tissues, with especially high levels of expression in liver, kidney, crop, and trachea, whereas AvBD10 was only expressed in the liver and kidney of ducks at all the ages investigated. We produced and purified GST-tagged recombinant AvBD9 and AvBD10 by expressing the two genes in Escherichia coli. Both recombinant proteins exhibited antimicrobial activity against several bacterial strains. The results revealed that both recombinant proteins retained their antimicrobial activities against Staphylococcus aureus under a range of different temperatures (-70 degrees C - 100 degrees C) and pH values (pH 3-12).
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