Molecular cloning and characterization of a T24-like protein in Echinococcus multilocularis

文献类型： 外文期刊

第一作者： Dang, Zhisheng

作者： Dang, Zhisheng;Kajino, Kiichi;Sugimoto, Chihiro;Dang, Zhisheng;Watanabe, Junichi;Oku, Yuzaburo;Matsumoto, Jun;Yagi, Kinpei;Kouguchi, Hirokazu

作者机构：

关键词： Echinococcus multilocularis;Metacestode;E24;Tetraspanin;Immunolocalization;Germinal layer

期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）

ISSN： 0166-6851

年卷期： 2009 年 168 卷 1 期

页码：

收录情况： SCI

摘要： One tetraspanin, designated as E24, was cloned from a full-length enriched vector-capping cDNA library of Echinococcus multilocularis metacestode. The amino acid sequence and phylogenetic analysis suggested that E24 is a T24-like protein. The crucial, functional large extracellular loop (LEL) domain of E24 was expressed and characterized using a polyclonal antiserum by Western blot and immunohistochemistry. The results showed that anti-recombinant-E24 (anti-recE24) antibody can specifically recognize approximately 25 kDa recombinant protein and 25 kDa cyst-extracted antigen; the germinal layer of both the protoscolex-free and protoscolex-formed cysts were intensely labeled by immunofluorescent antibody. This study revealed that E24 is an antigenic. germinal layer-located protein of E. multilocularis metacestode, implying for its potential in diagnostic and vaccine development. (C) 2009 Elsevier B.V. All rights reserved.

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