Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.

文献类型： 外文期刊

第一作者： Chao, Yuehui

作者： Chao, Yuehui;Kang, Junmei;Yang, Qingchuan;Wu, Mingsheng;Li, Yan;Chao, Yuehui;Wang, Pingqing;Long, Ruicai;Qin, Zhihui;Sun, Yan

作者机构：

关键词： Medicago sativa L.;Cloning;Zinc finger protein;Subcellular localization;Expression

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2009 年 36 卷 8 期

页码：

收录情况： SCI

摘要： A suppression subtraction hybridization (SSH) cDNA library had been constructed to identify differentially expressed genes. Based on the sequence of an expressed sequence tag (EST) homologous to Pisum sativum zinc finger protein mRNA (Accession number: AF160911), the full-length cDNA of 1,676 nucleotides was cloned from alfalfa by rapid amplification of cDNA ends (RACE). It was designated as MsZFN, encoding a protein of 418 amino acids. The amino acid sequence compared by blast revealed high homology with zinc finger protein of other plants. Sequence comparison showed that there were five conserved typical zinc finger motifs, and one sugar transfer protein signature. The calculated molecular weight of the MsZFN protein was 45.8 k Da, and theoretical isoelectric point was 8.13. The MsZFN localized in nucleus. Under normal growth conditions, differential expression of MsZFN exhibited that the expression was the highest in leaf and the lowest in root. MsZFN was quickly and transiently induced by NaCl treatment and reached its maximum at 30 min.

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