Identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks (Retracted article. See vol. 51, pg. 273, 2013)
文献类型: 外文期刊
第一作者: Wang, Ruiqin
作者: Wang, Ruiqin;Liao, Wenyan;Han, Zongxi;Liu, Shengwang;Ma, Deying;Wang, Ruiqin;Liao, Wenyan
作者机构:
关键词: duck;AvBD;recombinant protein;antimicrobial activity
期刊名称:JOURNAL OF MICROBIOLOGY ( 影响因子:3.422; 五年影响因子:3.28 )
ISSN: 1225-8873
年卷期: 2009 年 47 卷 5 期
页码:
收录情况: SCI
摘要: In this study, a novel avian beta-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20A degrees C to 100A degrees C) and pH values (range, 3 to 12).
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