Identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks (Retracted article. See vol. 51, pg. 273, 2013)
文献类型: 外文期刊
第一作者: Wang, Ruiqin
作者: Wang, Ruiqin;Liao, Wenyan;Han, Zongxi;Liu, Shengwang;Ma, Deying;Wang, Ruiqin;Liao, Wenyan
作者机构:
关键词: duck;AvBD;recombinant protein;antimicrobial activity
期刊名称:JOURNAL OF MICROBIOLOGY ( 影响因子:3.422; 五年影响因子:3.28 )
ISSN: 1225-8873
年卷期: 2009 年 47 卷 5 期
页码:
收录情况: SCI
摘要: In this study, a novel avian beta-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20A degrees C to 100A degrees C) and pH values (range, 3 to 12).
分类号:
- 相关文献
作者其他论文 更多>>
-
Newcastle disease virus activates diverse signaling pathways via Src to facilitate virus entry into host macrophages
作者:Shi, Qiankai;Zhao, Ran;Chen, Linna;Liu, Tianyi;Di, Tao;Zhang, Chunwei;Zhang, Zhiying;Wang, Fangfang;Han, Zongxi;Sun, Junfeng;Liu, Shengwang
关键词:Newcastle disease virus; gangliosides; Src; virus entry
-
Integrative RNA-seq and ChIP-seq analysis unveils metabolic regulation as a conserved antiviral mechanism of chicken p53
作者:Cui, Lu;Li, Xuefeng;Chen, Zhijie;Liu, Zheyi;Zhang, Yu;Han, Zongxi;Liu, Shengwang;Li, Hai
关键词:chicken; p53; metabolism; ChIP-seq; RNA-seq
-
Simultaneous Detection of Three Subgroups of Avian Leukosis Virus Using the Nanoparticle-Assisted PCR Assay
作者:Wu, Miaoli;Liu, Shengwang;Wu, Miaoli;Zhu, Yujun;Cong, Feng;Wu, Miaoli;Zhu, Yujun;Cong, Feng;Hu, Shuaiqi;Hu, Shuaiqi
关键词:nanoPCR; avian leukosis virus; discrimination
-
MAPK pathway orchestrates gallid alphaherpesvirus 1 infection through the biphasic activation of MEK/ERK and p38 MAPK signaling
作者:Cui, Lu;Li, Xuefeng;Liu, Zheyi;Zhang, Yu;Han, Zongxi;Liu, Shengwang;Li, Hai;Li, Xuefeng;Liu, Xiaoxiao;Zhu, Yongxin;Zhang, Yilei;Li, Hai
关键词:Mitogen-activated protein kinase (MAPK); Herpesvirus; Extracellular signal-regulated kinase (ERK); p38 MAPK; Apoptosis; Cell metabolism
-
Development of a Recombinant Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies Against Infectious Bronchitis Virus
作者:Zhang, Yu;Han, Zongxi;Li, Huixin;Liu, Shengwang
关键词:infectious bronchitis virus; nucleocaspid protein; recombinant antigen; ELISA
-
Effect of oral vitamin A supplementation on host immune response to infectious bronchitis virus infection in specific pathogen-free chicken
作者:Zhang, Lili;Hou, Yutong;Ma, Zhanbang;Xie, Jinjin;Fan, Jiahui;Jiao, Yaru;Wang, Fangfang;Ma, Deying;Zhang, Lili;Hou, Yutong;Ma, Zhanbang;Xie, Jinjin;Fan, Jiahui;Jiao, Yaru;Wang, Fangfang;Han, Zongxi;Liu, Shengwang
关键词:vitamin A; infectious bronchitis virus; specific pathogen-free chickens; IgG; viral load
-
Pathogenicity and innate immune responses induced by fowl adenovirus serotype 8b in specific pathogen-free chicken
作者:Zhang, Xiaona;Liu, Liangliang;Fan, Jiahui;Xie, Jingjing;Jiao, Yaru;Ma, Deying;Zhang, Xiaona;Liu, Liangliang;Wang, Fangfang;Li, Huixin;Fan, Jiahui;Xie, Jingjing;Jiao, Yaru;Han, Zongxi
关键词:fowl adenovirus serotype 8b; speci fic pathogen-free chicken; phathogenicity; immune response