Determination of tryptophan in bee pollen and royal jelly by high-performance liquid chromatography with fluorescence detection

文献类型： 外文期刊

第一作者： Zhang, Jin-zhen

作者： Zhang, Jin-zhen;Xue, Xiao-feng;Zhou, Jin-hui;Chen, Fang;Wu, Li-ming;Li, Yi;Zhao, Jing

作者机构：

关键词： tryptophan;HPLC;fluorescence detection;bee pollen;royal jelly

期刊名称：BIOMEDICAL CHROMATOGRAPHY （ 影响因子：1.902； 五年影响因子：1.809 ）

ISSN： 0269-3879

年卷期： 2009 年 23 卷 9 期

页码：

收录情况： SCI

摘要： A method for tryptophan analysis in bee pollen and royal jelly was developed using HPLC with fluorescence detection. To determine the free tryptophan in bee pollen and royal jelly, ultrasonic extraction was performed using water (pH 6.3)-acetonitrile (10:1, v/v) as extraction solvent. While determining the total tryptophan in these bee products, the method involves alkaline hydrolysis of the proteins with 4 mol/L sodium hydroxide at 110 degrees C for 20 h under anaerobic conditions. The operating conditions for the HPLC analysis were: Symmetry C(18) column (4.6 x 250 mm, 5 mu m), 0.1% trifluoroacetic acid-methanol (75:25, v/v) as the mobile phase at a flow rate of 1.0 mL/min at 30 degrees C. The fluorescence detector was operated at an excitation wavelength of 280 nm and an emission wavelength of 340 nm. A linear response (r(2) > 0.9998) was obtained in the range 0.0625-5.0 mu g/mL. The method was successfully applied to the determination of the free and total tryptophan contents in bee pollens, which were 0.069 +/- 0.003 and 2.693 +/- 0.476 mg/g, respectively, while only the total tryptophan was detected in royal jelly, with a content of 1.743 +/- 0.066 mg/g. Copyright (C) 2009 John Wiley & Sons, Ltd.

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