Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco

文献类型： 外文期刊

第一作者： Zhang, Gaiyun

作者： Zhang, Gaiyun;Chen, Ming;Li, Liancheng;Xu, Zhaoshi;Ma, Youzhi;Zhang, Gaiyun;Chen, Xueping;Guo, Jiaming

作者机构：

关键词： Abiotic stress;biotic stress;ethylene response factor;pathogen

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2009 年 60 卷 13 期

页码：

收录情况： SCI

摘要： A new member of the AP2/ERF transcription factor family, GmERF3, was isolated from soybean. Sequence analysis showed that GmERF3 contained an AP2/ERF domain of 58 amino acids and two putative nuclear localization signal (NLS) domains. It belonged to a group IV protein in the ERF (ethylene response factor) subfamily as typified by a conserved N-terminal motif [MCGGAI(I/L)]. Expression of GmERF3 was induced by treatments with high salinity, drought, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), ethylene (ET), and soybean mosaic virus (SMV), whereas there was no significant GmERF3 mRNA accumulation under cold stress treatment. GmERF3 could bind to the GCC box and DRE/CRT element, and was targeted to the nucleus when transiently expressed in onion epidermal cells. The GmERF3 protein fused to the GAL4 DNA-binding domain to activate transcription of reporter genes in yeast. Ectopic expression of the GmERF3 gene in transgenic tobacco plants induced the expression of some PR genes and enhanced resistance against infection by Ralstonia solanacearum, Alternaria alternata, and tobacco mosaic virus (TMV), and gave tolerance to high salinity and dehydration stresses. Furthermore, overexpression of GmERF3 in transgenic tobacco led to higher levels of free proline and soluble carbohydrates compared to wild-type plants under drought conditions. The overall results suggested that GmERF3 as an AP2/ERF transcription factor may play dual roles in response to biotic and abiotic stresses in plants.

分类号：

  • 相关文献

[1]Identification and characterization of reverse transcriptase domain of mranscriptionally active retrotransposons in wheat genomes. Tang, YM,Ma, YZ,Li, LC,Ye, XG. 2005

[2]Identification and expression profiling analysis of calmodulin-binding transcription activator genes in maize (Zea mays L.) under abiotic and biotic stresses. Yue, Runqing,Lu, Caixia,Han, Xiaohua,Qi, Jianshuang,Yan, Shufeng,Tie, Shuanggui,Yue, Runqing,Lu, Caixia,Han, Xiaohua,Qi, Jianshuang,Yan, Shufeng,Tie, Shuanggui,Sun, Tao,Peng, Tingting. 2015

[3]Expansins: roles in plant growth and potential applications in crop improvement. Marowa, Prince,Ding, Anming,Kong, Yingzhen.

[4]Molecular cloning and expression analysis of eight calcium-dependent protein kinase (CDPK) genes from banana (Musa acuminata L. AAA group, cv. Cavendish). Wang, Z.,Li, J.,Jia, C.,Xu, B.,Jin, Z.,Jin, Z..

[5]A comparative study of stress-related gene expression under single stress and intercross stress in rice. Zhang, Y. P.,E, Z. G.,Wang, L.,Zhu, D. F.,Jiang, H.,Zhou, J.. 2015

[6]Isolation and characterization of BnMKK1 responsive to multiple stresses and affecting plant architecture in tobacco. Yu, Shunwu,Chen, Chen,Li, Jiajia,Ye, Shuifeng,Liu, Guolan,Mei, Xiaohan,Luo, Lijun,Zhang, Lida,Tang, Kexuan.

[7]The AGPase Family Proteins in Banana: Genome-Wide Identification, Phylogeny, and Expression Analyses Reveal Their Involvement in the Development, Ripening, and Abiotic/Biotic Stress Responses. Miao, Hongxia,Liu, Juhua,Xu, Biyu,Jin, Zhiqiang,Sun, Peiguang,Jin, Zhiqiang,Liu, Qing. 2017

[8]Molecular cloning and expression of five glutathione S-transferase (GST) genes from Banana (Musa acuminata L. AAA group, cv. Cavendish). Wang, Zhuo,Huang, Suzhen,Jia, Caihong,Liu, Juhua,Zhang, Jianbin,Xu, Biyu,Jin, Zhiqiang. 2013

[9]Isolation, Phylogeny and Expression Patterns of AP2-Like Genes in Apple (Malus x domestica Borkh). Zhuang, Jing,Yao, Quan-Hong,Xiong, Ai-Sheng,Zhuang, Jing,Zhang, Jian.

[10]Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings. Dong, Chun-Juan,Wang, Xiao-Li,Shang, Qing-Mao.

[11]Genome-wide analysis of the RING finger gene family in apple. Li, Yanze,Wu, Bingjiang,Yang, Guodong,Wu, Changai,Zheng, Chengchao,Yu, Yanli.

[12]An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato. Pan, Yu,Hu, Zongli,Chen, Guoping,Pan, Yu,Seymour, Graham B.,Lu, Chungui,Chen, Xuqing. 2012

[13]Ethylene response factor BnERF2-like (ERF2.4) from Brassica napus L. enhances submergence tolerance and alleviates oxidative damage caused by submergence in Arabidopsis thaliana. Lv, Yanyan,Fu, Sanxiong,Chen, Song,Zhang, Wei,Qi, Cunkou,Qi, Cunkou. 2016

[14]A novel activator-type ERF of Thinopyrum intermedium, TiERF1, positively regulates defence responses. Liang, HongXia,Lu, Yan,Liu, HongXia,Wang, FengDe,Xin, ZhiYong,Zhang, ZengYan. 2008

[15]Regulation of plant stress response by dehydration responsive element binding (DREB) transcription factors. Zhou, Mei-Liang,Ma, Jiang-Tao,Pang, Jun-Feng,Tang, Yi-Xiong,Wu, Yan-Min,Ma, Jiang-Tao,Pang, Jun-Feng,Zhang, Zhan-Lu,Zhou, Mei-Liang. 2010

[16]The Wheat Bax Inhibitor-1 Protein Interacts with an Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis. Lu, Pan-Pan,Yu, Tai-Fei,Zheng, Wei-Jun,Zhou, Yong-Bin,Xi, Ya-Jun,Lu, Pan-Pan,Yu, Tai-Fei,Chen, Ming,Zhou, Yong-Bin,Chen, Jun,Ma, You-Zhi,Xu, Zhao-Shi. 2018

[17]Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.). Yin, Yan-Xu,Zhang, Huai-Xia,Xiao, Huai-Juan,Jin, Jing-Hao,Ji, Jiao-Jiao,Jing, Hua,Chen, Ru-Gang,Arisha, Mohamed Hamed,Gong, Zhen-Hui,Wang, Shu-Bin,Arisha, Mohamed Hamed.

[18]Identification of six mitogen-activated protein kinase (MAPK) genes in banana (Musa acuminata L. AAA group, cv. Cavendish) under infection of Fusarium Oxysporum f. sp cubense Tropical Race 4. Wang, Zhuo,Jia, Caihong,Li, Jianpin,Xu, Biyu,Jin, Zhiqiang,Jin, Zhiqiang.

[19]Identification of genes potentially related to herbivore resistance in OPR3 overexpression rice by microarray analysis. Guo, Hui-Min,Miao, Xue-Xia,Sun, Shi-Chen,Zhang, Feng-Ming.

[20]Proteomic dissection of plant responses to various pathogens. Fang, Xianping,Chen, Jianping,Dai, Liangying,Fang, Xianping,Chen, Jianping,Dai, Liangying,Fang, Xianping,Chen, Jianping,Zhang, Hengmu,Yang, Jian,Yan, Chengqi,Fang, Xianping,Ma, Huasheng,Wang, Fang.

作者其他论文 更多>>

微信小程序