Gene cloning, expression and characterization of an alpha-galactosidase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive

文献类型： 外文期刊

第一作者： Liu, Xiaodan

作者： Liu, Xiaodan;Meng, Kun;Wang, Yaru;Shi, Pengjun;Yuan, Tiezheng;Yang, Peilong;Luo, Huiying;Bai, Yingguo;Yao, Bin;Liu, Xiaodan

作者机构：

关键词： alpha-Galactosidase;Pedobacter nyackensis;Cloning;Expression

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2009 年 25 卷 9 期

页码：

收录情况： SCI

摘要： A gene, aga-MJ11, encoding an alpha-galactosidase (EC 3.2.1.22) was cloned from Pedobacter nyackensis MJ11 CGMCC 2503, expressed in Escherichia coli, and biochemically characterized. The gene consisted of 2,163 nucleotides encoding a 720 amino acid-protein with a theoretical molecular weight of 82.6 kDa. The deduced amino acid sequence of Aga-MJ11 shared the highest identity of 51% to an alpha-galactosidase from Parabacteroides distasonis (YP_001301506), which belongs to glycoside hydrolase (GH) family 36. Purified recombinant Aga-MJ11-H showed optimal activity at pH 5.5 and 40A degrees C, was stable at pH 4.0-10.0, retained similar to 80% of the maximum activity at 30A degrees C (the optimum temperature for freshwater fish), exhibited tolerance to some proteases, and had a wide substrate specificity (pNPG, melidiose, stachyose and raffinose). All these features make Aga-MJ11 potentially useful for applications in aquaculture. The enzyme studied in the present work may represent a novel GH-36 alpha-galactosidase from the genus Pedobacter.

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