Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus

文献类型： 外文期刊

第一作者： Wu, Ping

作者： Wu, Ping;Li, Mu-Wang;Wang, Xiu-Ye;Liu, Ting;Qin, Guang-Xing;Guo, Xi-Jie;Wang, Xiu;Zhao, Pan;Guo, Xi-Jie

作者机构：

关键词： Bombyx mori;cytoplasmic polyhedrosis virus;midgut;suppression subtractive hybridization;quantitative real-time PCR

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2009 年 8 卷 16 期

页码：

收录情况： SCI

摘要： Understanding of the responsive and interactive mechanism between the host cells and Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is crucial to the diagnosis of CPV-caused disease and the development of new control measures. In this report, we employed suppression subtractive hybridization to compare differentially expressed genes in the midguts of CPV-infected and normal silkworm larvae. 36 genes and 20 novel ESTs were obtained from 2 reciprocal subtractive libraries. Three up-regulated genes (ferritin, rpL11 and alkaline nuclease) and 3 down-regulated genes (serine protease, trypsin-like protease and inhibitor of apoptosis protein) were identified by quantitative real-time PCR. The transcript differences of these 6 genes at 6, 12, 24, 48 and 72 h post-inoculation both in CPV-infected and normal midguts were compared. Our results indicated that ferritin and rpL11 were increased during the early stage (6-12h p.i.) of CPV infection, whereas alkaline nuclease was increased during the late stage (24-72h p.i.) of CPV infection. The expression of serine protease and trypsin-like protease is decreased at 24-72 h after CPV infection, while the expression of inhibitor of apoptosis protein is decreased throughout the infective stage. Our results provide new clues for investigating the molecular mechanism of BmCPV infection.

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