Immune responses induced by a BacMam virus expressing the E2 protein of classical swine fever virus in mice

文献类型： 外文期刊

第一作者： Li, Miao

作者： Li, Miao;Wang, Yu-Fei;Wang, Yu;Gao, Hui;Li, Na;Sun, Yuan;Liang, Bing-Bing;Qiu, Hua-Ji

作者机构：

关键词： Classical swine fever virus;E2 gene;BacMam virus;Recombinant baculovirus;Immunogenicity

期刊名称：IMMUNOLOGY LETTERS （ 影响因子：3.685； 五年影响因子：3.54 ）

ISSN： 0165-2478

年卷期： 2009 年 125 卷 2 期

页码：

收录情况： SCI

摘要： Non-replicating baculovirus-mediated gene transfer into mammalian cells has been developed as a vaccine strategy against a number of diseases in several animal models. In the present study. the BacMam vector, a baculovirus pseudotyped with the glycoprotein from vesicular stomatitis virus, was used as a recombinant vector to express classical swine fever virus (CSFV) E2 protein under the control of the immediate early 1 (ie1) promoter from shrimp white spot syndrome virus. The E2 gene was efficiently expressed in both insect and mammalian cells. Intramuscular injection of mice with the recombinant baculovirus resulted in the production of high-titers of CSFV-specific neutralizing antibodies. Specific lymphoproliferative responses to CSFV stimulation were detected in the splenocytes of the immunized mice as demonstrated by USE staining assay and WST-8 assay. This study demonstrates that the BacMam virus vector can efficiently express the E2 protein and effectively induce immune responses against CSFV. This is a first step in the demonstration that the pseudotyped baculovirus-delivered CSFV E2 gene can be a potential non-replicating vaccine against CSFV infections. (C) 2009 Elsevier B.V. All rights reserved.

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