文献类型: 外文期刊
第一作者: Cai, Li
作者: Cai, Li;Hou, Mingsheng;Cai, Li;Chen, Kunrong;Zhang, Xuejiang;Yan, Liying;Xu, Zeyong
作者机构:
关键词: Youcai mosaic virus (YoMV);Serology;Sequence;Phylogenetic analysis
期刊名称:BIOCHEMICAL GENETICS ( 影响因子:1.89; 五年影响因子:1.959 )
ISSN: 0006-2928
年卷期: 2009 年 47 卷 7-8 期
页码:
收录情况: SCI
摘要: In China, the tobamovirus that infects oilseed rape has been misdiagnosed as Tobacco mosaic virus (TMV) based on its morphological similarity and serological relatedness. Recently, a tobamovirus has been isolated from oilseed rape in China, which we named Youcai mosaic virus Br (YoMV-Br), according to its biological and molecular characteristics. It had strong infectivity to Cruciferae but less to Solanaceae, Leguminosae, and Cucurbitaceae, and its virion morphology was consistent with that of the tobamoviruses. At high concentrations, it serologically cross reacted with TMV antiserum. The 3' terminal sequence (2,283 nucleotides) of YoMV-Br was determined, including the 3' noncoding region, the CP and MP genes, and the C-terminal part of the replicase gene. Between the MP and CP genes, 77 nucleotides overlapped. Compared with homologous regions of 21 recognized species of Tobamovirus, YoMV-Br had a much higher identity to crucifer species than to other tobamoviruses. Phylogenetic analysis demonstrated that YoMV-Br was closely related to the YoMV cluster of tobamoviruses and distantly to TMV, so that they likely belong to different strains of the same species.
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