A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity

文献类型： 外文期刊

第一作者： Sun AiJun

作者： Sun AiJun;Cui ZhiZhong;Lawrence, Petherbridge;Zhao YuGuang;Nair, Venugopal K.;Li YanPeng

作者机构：

关键词： Marek's disease virus;bacterial artificial chromosome;reticuloendotheliosis virus-LTR

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2009 年 54 卷 15 期

页码：

收录情况： SCI

摘要： The complete genome of Marek's disease virus (MDV) strain GX0101, which was integrated with the LTR sequences of REV, was cloned in Escherichia coli as a bacterial artificial chromosome (BAC). BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination. The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B. Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF). This BAC viral clone was named bac-GX0101. When the reconstituted virus was inoculated into 1-day-old birds, visceral tumors could be detected as early as 62 d post infection. There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus. The BAC derived virus maintained its oncogenicity and immunosuppressive effects. In conclusion, the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued. With the powerful BAC manipulation system, the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV.

分类号：

  • 相关文献

[1]An inactivated gE-deleted pseudorabies vaccine provides complete clinical protection and reduces virus shedding against challenge by a Chinese pseudorabies variant. Wang, Jichun,Guo, Rongli,Qiao, Yongfeng,Xu, Mengwei,Wang, Zhisheng,Liu, Yamei,Gu, Yiqi,Liu, Chang,Hou, Jibo,Gu, Yiqi,Liu, Chang,Wang, Jichun,Guo, Rongli,Qiao, Yongfeng,Xu, Mengwei,Wang, Zhisheng,Liu, Yamei,Hou, Jibo. 2016

[2]Construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain. Chen, Liu,Yu, Bin,Hua, Jionggang,Ye, Weicheng,Ni, Zheng,Yun, Tao,Deng, Xiaohui,Zhang, Cun. 2013

[3]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[4]Identification of sesame (Sesamum indicum L.) chromosomes using the BAC-FISH system. Zhao, R.,Song, W.,Chen, C.,Zhao, R.,Miao, H.,Zhang, H.. 2018

[5]Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus. Guo, Jin-Chao,Tang, Yan-Dong,Zhao, Kuan,Wang, Tong-Yun,Liu, Ji-Ting,Gao, Jia-Cong,Chang, Xiao-Bo,Cui, Hong-Yu,Tian, Zhi-Jun,Can, Xue-Hui,An, Tong-Qing. 2016

[6]Cloning of equine herpesvirus type 1 438/77 strain genome as a bacterial artificial chromosome. Sun, Xuegiang,Yao, Huochun,Lu, Chengping,Zhang, Cun.

[7]Fine mapping of a gene responsible for pollen semi-sterility in hybrids between Oryza sativa L. and O. glaberrima Steud. Wang, Jiankang,Wan, Jianmin,Zhang, Yunhui,Zhao, Zhigang,Zhou, Jiawu,Jiang, Ling,Bian, Xiaofeng,Wang, Yong,Wang, Chaolong,Zhong, Zhengzheng,Wan, Jianmin,Zhou, Jiawu,Tao, Dayun.

[8]Co-Infection with Marek's Disease Virus and Reticuloendotheliosis Virus Increases Illness Severity and Reduces Marek's Disease Vaccine Efficacy. Sun, Guo-Rong,Zhang, Yan-Ping,Zhou, Lin-Yi,Lv, Hong-Chao,Zhang, Feng,Li, Kai,Gao, Yu-Long,Qi, Xiao-Le,Cui, Hong-Yu,Wang, Yong-Qiang,Gao, Li,Pan, Qing,Wang, Xiao-Mei,Liu, Chang-Jun. 2017

[9]Primary studies on immunoprotectivity of Marek's disease virus DNA vaccine. Li, JW,Zhao, XY,Yang, JD,Liu, CJ,Li, X,Tian, ZJ,Xu, YW. 1996

[10]Depression of Vaccinal Immunity to Marek's Disease by Infection with Chicken Infectious Anemia Virus. Zhang, Yankun,Cui, Ning,Han, Ni,Wu, Jiayan,Cui, Zhizhong,Su, Shuai,Zhang, Yankun,Cui, Ning,Han, Ni,Wu, Jiayan,Su, Shuai,Zhang, Yankun,Cui, Ning,Han, Ni,Wu, Jiayan,Su, Shuai,Cui, Ning. 2017

[11]A Tiny RNA that Packs a Big Punch: The Critical Role of a Viral miR-155 Ortholog in Lymphomagenesis in Marek's Disease. Zhuang, Guoqing,Sun, Aijun,Teng, Man,Luo, Jun,Luo, Jun. 2017

[12]Pathogenic characteristics of Marek's disease virus field strains prevalent in China and the effectiveness of existing vaccines against them. Zhang, Yan-ping,Wang, Xiao-mei,Zhang, Yan-ping,Li, Zhi-jie,Bao, Ke-yan,Lv, Hong-chao,Gao, Yu-long,Gao, Hong-lei,Qi, Xiao-le,Cui, Hong-yu,Wang, Yong-qiang,Ren, Xian-gang,Wang, Xiao-mei,Liu, Chang-jun.

[13]Protection of Chickens against Very Virulent Marek's Disease Virus (MDV) by an Infectious Clone of Meq-Null MDV Vaccination. Cui, Ning,Su, Shuai,Cui, Zhizhong,Sun, Peng,Li, Yanpeng,Zhu, Hongfei,Ding, Jiabo,Kang, Mengjiao.

[14]Comparison of different sites in recombinant Marek's disease virus for the expression of green fluorescent protein. Liu, Yongzhen,Li, Kai,Cui, Hongyu,Gao, Li,Liu, Changjun,Zhang, Yanping,Gao, Yulong,Wang, Xiaomei.

[15]Isolation and full-genome sequence of two reticuloendotheliosis virus strains from mixed infections with Marek's disease virus in China. Bao, Ke-yan,Zhang, Yan-ping,Zheng, Hui-wen,Lv, Hong-chao,Gao, Yu-long,Wang, Jing-fei,Gao, Hong-lei,Qi, Xiao-le,Cui, Hong-yu,Wang, Yong-qiang,Ren, Xian-gang,Wang, Xiao-mei,Liu, Chang-jun,Wang, Xiao-mei.

[16]Deletion of the BAC sequences from recombinant meq-null Marek's disease (MD) virus increases immunosuppression while maintaining protective efficacy against MD. Su, S.,Cui, N.,Sun, P.,Cui, Z.,Li, J.,Li, H.,Li, Y..

[17]Evaluation of two strains of Mareles disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Gao, Yulong,Zhang, Yanping,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[18]A recombinant field strain of Marek's disease (MD) virus with reticuloendotheliosis virus long terminal repeat insert lacking the meq gene as a vaccine against MD. Su, Shuai,Cui, Ning,Chen, Zimeng,Wang, Yixin,Duan, Luntao,Cui, Zhizhong,Zhou, Yu,Li, Yanpeng,Ding, Jiabo.

[19]Development of a rapid and specific loop-mediated isothermal amplification detection method that targets Marek's disease virus meq gene. Wei, Xiuying,Shi, Xingming,Zhao, Yan,Zhang, Jing,Wang, Mei,Liu, Changjun,Cui, Hongyu,Hu, Shunlei,Quan, Yanming,Chen, Hongyan,Wang, Yunfeng,Wei, Xiuying. 2012

[20]Sequence analysis of the Meq gene in the predominant Marek's disease virus strains isolated in China during 2006-2008. Zhang, Yan-ping,Liu, Chang-jun,Zhang, Feng,Shi, Weisong,Li, Jingmei. 2011

作者其他论文 更多>>

微信小程序