Identification and antifungal assay of a wheat beta-1,3-glucanase
文献类型: 外文期刊
第一作者: Liu, Baoye
作者: Liu, Baoye;Lu, Yan;Xin, Zhiyong;Zhang, Zengyan
作者机构:
关键词: Antifungal activity;beta-1,3-Glucanase;Expression;Rhizoctonia cerealis;Triticum aestivum
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN: 0141-5492
年卷期: 2009 年 31 卷 7 期
页码:
收录情况: SCI
摘要: A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
分类号:
- 相关文献
作者其他论文 更多>>
-
TaRLK-6A promotes Fusarium crown rot resistance in wheat
作者:Qi, Haijun;Qi, Haijun;Zhu, Xiuliang;Wei, Xuening;Zhang, Zengyan;Qi, Haijun;Shen, Wenbiao;Yang, Xia;Chen, Feng;Zhang, Chaozhong;Li, Genying
关键词:
-
Novel xanthone antibacterials: Semi-synthesis, biological evaluation, and the action mechanisms
作者:Lu, Yan;Zhou, Cui;Wang, Meizhu;Wang, Xiaoyang;Zhang, Keyu;Han, Xiangan;Tang, Qun;Wang, Chunmei;Zhou, Wen;Lu, Yan;Wang, Shaobing;Guan, Ting;Lin, Jinchao;Wang, Xiaoyang;Zhang, Keyu;Tang, Qun;Wang, Chunmei;Zhou, Wen
关键词:alpha-Mangostin derivatives; Synthesis; Staphylococcus aureus; Structure activity relationships; Antibacterial mechanism
-
A Glu209Lys substitution in DRG1/TaACT7, which disturbs F-actin organization, reduces plant height and grain length in bread wheat
作者:Xie, Zhencheng;Zhang, Lichao;Zhang, Qiang;Lu, Yan;Dong, Chunhao;Li, Danping;Liu, Xu;Xia, Chuan;Kong, Xiuying
关键词:actin; grain shape; map-based cloning; plant height; wheat
-
Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo
作者:Meng, Shun Long;Li, Ming Xiao;Lu, Yan;Chen, Xi;Song, Chao;Xu, Pao;Meng, Shun Long;Li, Ming Xiao;Chen, Xi;Song, Chao;Qiu, Li Ping;Li, Dan Dan;Xu, Hui Min;Xu, Pao;Meng, Shun Long;Li, Ming Xiao;Chen, Xi;Song, Chao;Qiu, Li Ping;Li, Dan Dan;Xu, Hui Min;Xu, Pao;Wang, Wei Ping
关键词:Methomyl; Ecotoxicology; Zebrafish embryo; Embryo morphology; Develop associated genes
-
The effect of high altitude on ephedrine content and metabolic variations in two species of Ephedra
作者:Lu, Mengnan;De, Ji;He, Wenjia;Xu, Ziyan;Lu, Yan;Crabbe, M. James C.;Crabbe, M. James C.;Crabbe, M. James C.
关键词:Ephedra; pseudoephedrine; ephedrine; metabolomics; altitude; environment
-
The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against Fusarium pseudograminearum in Wheat
作者:Xiong, Feng;Luo, Changsha;Liu, Zhixiang;Xiong, Feng;Zhu, Xiuliang;Zhang, Zengyan
关键词:cytosolic acetoacetyl-CoA thiolase; Fusarium pseudograminearum; jasmonate; plant defense; wheat (Triticum aestivum)
-
Genome sequencing reveals the evolution and pathogenic mechanisms of the wheat sharp eyespot pathogen Rhizoctonia cerealis
作者:Lu, Lin;Guo, Feilong;Zhu, Xiuliang;Hao, Yu;Zhang, Zengyan;Zhang, Zhichao;Ye, Wenwu;Hao, Yu;Yu, Jinfeng
关键词:Effector; Evolution; Pathogenesis; Rhizoctonia cerealis; Secretory proteins; Sharp eyespot; Wheat(Triticumaestivum)