Identification and antifungal assay of a wheat beta-1,3-glucanase

文献类型: 外文期刊

第一作者: Liu, Baoye

作者: Liu, Baoye;Lu, Yan;Xin, Zhiyong;Zhang, Zengyan

作者机构:

关键词: Antifungal activity;beta-1,3-Glucanase;Expression;Rhizoctonia cerealis;Triticum aestivum

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

ISSN: 0141-5492

年卷期: 2009 年 31 卷 7 期

页码:

收录情况: SCI

摘要: A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.

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