Identification and antifungal assay of a wheat beta-1,3-glucanase
文献类型: 外文期刊
第一作者: Liu, Baoye
作者: Liu, Baoye;Lu, Yan;Xin, Zhiyong;Zhang, Zengyan
作者机构:
关键词: Antifungal activity;beta-1,3-Glucanase;Expression;Rhizoctonia cerealis;Triticum aestivum
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN: 0141-5492
年卷期: 2009 年 31 卷 7 期
页码:
收录情况: SCI
摘要: A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
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