Determination of Nicotinamide in Rice by Pre-column Derivatization and High Performance Liquid Chromatography-Mass Spectrometry

文献类型: 外文期刊

第一作者: Cao Zhao-Yun

作者: Cao Zhao-Yun;Mou Ren-Xiang;Chen Ming-Xue

作者机构:

关键词: Nicotinamide;rice;pre-column derivatization;liquid chromatography-mass spectrometry

期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )

ISSN: 0253-3820

年卷期: 2009 年 37 卷 7 期

页码:

收录情况: SCI

摘要: A method was developed for the determination of nicotinamide (NA) in rice by pre-column derivatization and high performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS). After extraction with water, NA was derivatized by 9-fluorenylmethoxycarbonyl (FMOC-CI). In order to enhance the sensitivity, the derivatization was optimized regarding organic solvent content and amount of FMOC-Cl. The best HPLC conditions for the separation of NA in real samples were achieved using a C(18) column and mobile phase which contained 0.1% formic acid. The MS experiments were performed in selected ion monitoring mode (SIM). The method shows good linearity over the range of 0.1 - 5 mg/L for NA with a correlation coefficient of 0.9983. The limit of determination (LOD) was 0.05 mg/kg. Precision and recovery studies were evaluated at three concentration levels for rice root, stem, leaf and unpolished rice. The recovery (n = 5) ranged from 72.0% to 89.2% with relative standard deviations from 2.3% to 9.6%. The method is simple, highly selective and reliable, and can be Used to determine NA in rice at physiological level.

分类号:

  • 相关文献

[1]Quantification of piperazine in chicken and pig tissues by gas chromatography-electron ionization tandem mass spectrometry employing pre-column derivatization with acetic anhydride. Wang, Bo,Xie, Kaizhou,Zhang, Yangyang,Cui, Lulu,Zhao, Xia,Wang, Yajuan,Shi, Huiqiang,Guo, Yawen,Zhang, Genxi,Dai, Guojun,Wang, Jinyu,Wang, Bo,Xie, Kaizhou,Zhang, Yangyang,Cui, Lulu,Zhao, Xia,Wang, Yajuan,Shi, Huiqiang,Guo, Yawen,Zhang, Genxi,Dai, Guojun,Wang, Jinyu,Wang, Bo,Xie, Kaizhou,Zhang, Yangyang,Cui, Lulu,Zhao, Xia,Wang, Yajuan,Shi, Huiqiang,Guo, Yawen,Zhang, Genxi,Dai, Guojun,Wang, Jinyu,Pang, Maoda,Xie, Xing,Wang, Ran.

[2]Effect of SIRT1 on Apoptosis of Bovine Preadipocytes. Liu Xiao-Mu,Song En-Liang,Liu Gui-Fen,Tan Xiu-Wen,Liu Zhen-Shan,Wan Fa-Chun,Liu Xiao-Mu,Song En-Liang,Liu Gui-Fen,Tan Xiu-Wen,Liu Zhen-Shan,Wan Fa-Chun,Wu Gong-Ying. 2010

[3]Nicotinamide-based diamides derivatives as potential cytotoxic agents: synthesis and biological evaluation. Peng, Min,Shi, Liqiao,Ke, Shaoyong. 2017

[4]Effects of niacin status on tryptophan requirements of starter white Pekin ducks. Xie, M.,Huang, W.,Hon, S. S.,Han, X. F.. 2014

[5]An improved method for analyzing chlormequat and mepiquat in source waters by solid-phase extraction and liquid chromatography-mass spectrometry. Yu, Zhiyong,Sun, Jingfang,Yang, Min,Jin, Fen,Zhang, Xi,Hu, Jianying.

[6]Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping. Zhao, Jieyu,Li, Lili,Zhao, Yanni,Zhao, Chunxia,Zhang, Junjie,Hu, Chunxiu,Lu, Xin,Xu, Guowang,Zhao, Jieyu,Peng, Xiaojun,Chen, Xia,Liu, Pingping,Zhou, Huina,Chen, Aiguo,Liu, Guanshan. 2018

[7]Determination of Cortisol in Human Serum Using Ultrasonic Extraction Coupled with Liquid Chromatography-Isotope Dilution Mass Spectrometry. Zhou Jian,Wang Min,Tang Xiao-Yan,Mao Xue-Fei,Dai Xin-Hua. 2013

[8]Determination of Chlormequat in Muscles of Mice by Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometry. Li Chun-Mei,Jin Fen,Xu Si-Yuan,Liu Jia-Jia,Jin Mao-Jun,Shao Hua,Wang Jing,Li Chun-Mei,Yang Ming-Qi,Liu Jia-Jia. 2012

[9]Simultaneous determination of sulfonamides and metabolites in manure samples by one-step ultrasound/microwave-assisted solid-liquid-solid dispersive extraction and liquid chromatography-mass spectrometry. Wu, Hui-zhen,Li, Zu-guang,Wu, Hui-zhen,Qian, Ming-rong,Wang, Jian-mei,Zhang, Hu,Ma, Jun-wei,Lee, Maw-rong.

[10]Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice. He, Yanan,Li, Yaping,Cui, Lixin,Xie, Lixia,Zheng, Chongke,Zhou, Guanhua,Zhou, Jinjun,Xie, Xianzhi,Li, Yaping,Cui, Lixin. 2016

[11]A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice. Hu, Zejun,Sun, Fan,Xin, Xiaoyun,Qian, Xi,Yang, Jingshui,Luo, Xiaojin,Hu, Zejun,He, Haohua,Wang, Wenxiang,Zhang, Shiyong. 2012

[12]Genome-wide identification of microRNAs and their targets in wild type and phyB mutant provides a key link between microRNAs and the phyB-mediated light signaling pathway in rice. Sun, Wei,wu, Xiu,Xie, Xianzhi,Xu, Xiao Hui,Lu, Xingbo,Sun, Hongwei,Wang, Yong. 2015

[13]Overexpression of an S-like ribonuclease gene, OsRNS4, confers enhanced tolerance to high salinity and hyposensitivity to phytochrome-mediated light signals in rice. Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Li, Yaping,Liu, Qianqian,Xie, Xianzhi,Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Xie, Xianzhi. 2014

[14]Distribution Characteristics of Soil Cadmium in Different Textured Paddy Soil Profiles and Its Relevance with Cadmium Uptake by Crops. Wang Zheng-yin,Qin Yu-sheng,Zhan Shao-jun,Yu Hua,Tu Shi-hua. 2013

[15]Overexpression of OsPIL15, a phytochromeinteracting factor- like protein gene, represses etiolated seedling growth in rice. Zhou, Jinjun,Liu, Qianqian,Wang, Yingying,Zhang, Shiyong,Cheng, Huimin,Yan, Lihua,Li, Li,Xie, Xianzhi,Zhou, Jinjun,Wang, Yingying,Zhang, Shiyong,Xie, Xianzhi,Liu, Qianqian,Xie, Xianzhi,Zhang, Fang,Chen, Fan. 2014

[16]DISTRIBUTION CHARACTERISTICS, BIOACCUMULATION, AND SOURCES OF MERCURY IN RICE AT NANSI LAKE AREA, SHANDONG PROVINCE, CHNIA. Liu, H.,Zhang, J.,Dai, J. L.,Wang, L. H.,Zhang, J.,Li, G. X.. 2015

[17]Nitrogen Status Diagnosis of Rice by Using a Digital Camera. Fan Ming-sheng,Zhang Fu-suo,Chen Xin-ping,Jia Liang-liang,Sun Yan-ming,Lue Shi-hua. 2009

[18]Influence of unflooded mulching cultivation on nitrogen uptake and utilization of fertilizer nitrogen by rice. Liu, Xuejun,Zhang, Fusuo,Mao, Daru,Zeng, Xingzhong,Lu, Shihua,Wang, Mingtian. 2008

[19]Overexpression of a phytochrome-regulated tandem zinc finger protein gene, OsTZF1, confers hypersensitivity to ABA and hyposensitivity to red light and far-red light in rice seedlings. Zhou, Jinjun,Fan, Zhongxue,Xie, Xianzhi,Zhang, Cheng,Zhou, Jinjun,Fan, Zhongxue,Xie, Xianzhi,Zhang, Cheng,Ma, Huiquan,Zhang, Fang,Chen, Fan. 2012

[20]Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR. Lu, Fen,Wang, Huiqin,Wang, Shanzhi,Jiang, Wendi,Yang, Jun,Sun, Wenxian,Lu, Fen,Wang, Huiqin,Wang, Shanzhi,Jiang, Wendi,Yang, Jun,Sun, Wenxian,Shan, Changlin,Li, Bin,Shan, Changlin,Li, Bin,Yang, Jun,Zhang, Shiyong. 2015

作者其他论文 更多>>