Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus
文献类型： 外文期刊
第一作者： Lan, Xi
作者： Lan, Xi;Yang, Bin;Li, Bao Yu;Yin, Xiang Ping;Li, Xue Rui;Liu, Ji Xing
作者机构：
期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.948； 五年影响因子：5.345 ）
ISSN： 0095-1137
年卷期： 2009 年 47 卷 7 期
页码：
收录情况： SCI
摘要： The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).
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