Establishment and characterization of a fibroblast cell line derived from Texel sheep

文献类型： 外文期刊

第一作者： Li, Linfeng F.

作者： Li, Linfeng F.;Guan, Weijun J.;Li, Han;Zhou, Xueyan Z.;Ma, Yuehui H.;Li, Linfeng F.;Bai, Xiujuan J.

作者机构：

关键词： Texel sheep;fibroblast cell line;establishment;identification

期刊名称：BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE （ 影响因子：3.626； 五年影响因子：3.178 ）

ISSN： 0829-8211

年卷期： 2009 年 87 卷 3 期

页码：

收录情况： SCI

摘要： A Texel sheep ear marginal tissue fibroblast cell line (named TSF19) was successfully established by using a primary explant technique and cell cryoconservation technology. TSF19 cells were adherent, with a population doubling time of 24.9 h. Chromosome analysis showed that >90% of cells were diploid prior to cell passage 4. Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that the TSF19 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, or mycoplasmas were also negative. Plasmids encoding the fluorescent proteins pEGFP-N3, pECFP-N1, pDsRed1-N1, and pEYFP-N1 were transfected into cells to study exogenous gene expression in the cells. The plasmid transfection efficiency was between 21.8% and 46.5%. This newly established cell line will not only preserve the genetic resources of the important Texel sheep at the cell level but will also provide a valuable resource for genomic, postgenomic, somatic cloning research.

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