Fast determination of 26 amino acids and their content changes in royal jelly during storage using ultra-performance liquid chromatography

文献类型: 外文期刊

第一作者: Wu Liming

作者: Wu Liming;Zhou Jinhui;Xue Xiaofeng;Li Yi;Zhao Jing

作者机构:

关键词: UPLC;Free amino acid;Total amino acid;Royal jelly;Storage condition;Food composition

期刊名称:JOURNAL OF FOOD COMPOSITION AND ANALYSIS ( 影响因子:4.556; 五年影响因子:4.89 )

ISSN: 0889-1575

年卷期: 2009 年 22 卷 3 期

页码:

收录情况: SCI

摘要: A rapid ultra-performance liquid chromatography (UPLC) method was developed for feasible separation and quantification of 26 amino acids in royal jelly. The analysis was performed on Acquity UPLC system with Acquity UPLC AccQ-Tag Ultra Column within 8 min. The correlation coefficient values (r(2) > 0.9978) indicated good correlations between the investigated compounds' concentrations and their peak areas within the test ranges. The limits of quantitation and detection of 26 amino acids were 42.7-235.1 ng/mL and 12.9-69.3 ng/mL, respectively. The recoveries ranged from 90.1% to 100.9% and the overall relative standard deviations for intra- and inter-day were lower than 2.8%. The results showed that UPLC was a powerful tool for the analysis of amino acids in royal jelly. The method was also applied to quantitatively determine free amino acid (FAA) and total amino acid (TAA) profiles in RJ samples stored at different temperatures (-18 degrees C, 4 degrees C and 25 degrees C) for different time intervals (1, 3, 6 and 10 months). Results showed that the average contents of FAA and TAA in fresh royal jelly were 9.21 mg/g and 111.27 mg/g, respectively: the major FAAs were Pro, Gin, Lys, Glu, and the most abundant TAAs were Asp, Glu, Lys and Leu. Although the concentration of most FAAs and TAAs showed no significant difference during storage, contents of total Met and free Gln decreased significantly and continuously, and might be a parameter to predict the quality of royal jelly. (C) 2009 Elsevier Inc. All rights reserved.

分类号:

  • 相关文献

[1]Sensory, Physicochemical and Microbiological Changes in Water-cooked Salted Duck during Storage at 4 degrees C. Li, Yanliang,Yao, Dongrui,Wang, Daoying,Xu, Weimin,Zhu, Yongzhi.

[2]Oxidation stability of milk rich in alpha-linolenic acid produced through duodenum infusion of high-linolenic perilla fatty acid into dairy cows. Liu, Q. S.,Wang, J. Q.,Bu, D. P.,Khas, E.,Yang, G.,Zhou, L. Y.,Sun, P.,Liu, K. L.. 2010

[3]Proteome Analysis of Tea Pollen (Camellia sinensis) under Different Storage Conditions. Zhang, Zhaohui,Pan, Yinghong,Li, Jianke,Chen, Jian,Chen, Jian.

[4]Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties. Xi, Wanpeng,Fang, Bo,Zhou, Zhiqin,Zhao, Qiyang,Jiao, Bining,Xi, Wanpeng,Zhou, Zhiqin.

[5]A bioisosteres' application as internal standard for simultaneous determination of three triazine anticoccidial chemicals by UPLC. Ma, Danpeng,Xue, Feiqun,Zhang, Lifang,Fei, Chenzhong,Ma, Danpeng,Zhang, Yue.

[6]Fast Separation Ultra-Performance Liquid Chromatography for Determination of Pre-Column Derivative Abamectin and Ivermectin Residues in Vegetable. Li, Qiwan,Liu, Hongcheng,Li, Qiwan,Shao, Jinliang,Zou, Yanhong,Liu, Hongcheng,Zhang, Ying,Liu, Lianliang. 2011

[7]Impact of Genetic Factor and Geographical Location on Allicin Content of Garlic (Allium sativum) Germplasm from Egypt and China. Mostafa, Hassan H. A.,Wang Haiping,Liu Xinyan,Li Xixiang,Mostafa, Hassan H. A.. 2015

[8]Antibacterial properties of anthraquinones extracted from rhubarb against Aeromonas hydrophila. Lu, Chunxia,Wang, Hongxin,Lv, Wenping,Lou, Zaixiang,Xu, Pao,Zhu, Jian,Xie, Jun,Liu, Bo. 2011

[9]Quantification of lutein and zeaxanthin in marigold (Tagetes erecta L.) and poultry feed by ultra-performance liquid chromatography and high performance liquid chromatography. Liu, Hongcheng,Li, Qiwan,Zou, Yanhong,Shao, Jinliang,Lan, Shanshan,Liu, Hongcheng,Zhang, Ying.

[10]Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment. Qiu, Jian,Chen, Fudong,Zhao, Dexiu,Qiu, Jian,Chen, Fudong,Li, Chonghui,Zhao, Qiao,Xue, Xiaofeng,Bolat, Nuer,Wang, Xiaojun,Baima, Yuzhen,Ma, Fengshan.

[11]Development and validation of an UPLC-UV method for determination of a novel triazine coccidiostat ethanamizuril and its metabolite M3 in chicken tissues. Zhao, Xiao,Zhang, Lifang,Wang, Chunmei,Guo, Chunna,Fei, Chenzhong,Zhang, Keyu,Wang, Xiaoyang,Liu, Yingchun,Wang, Mi,Zheng, Wenli,Xue, Feiqun,Xu, Youfa.

[12]Determination of 21 Free Amino Acids in Fruit Juices by HPLC Using a Modification of the 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate (AQC) Method. Ou, Jun,Huang, Yufen,Li, Qian,Xu, Guizhi,Liu, Zhongzhen,Yang, Shaohai,Zeng, Fang.

[13]Postharvest changes in physicochemical characteristics and free amino acids content of immature vegetable soya bean (Glycine max L.) grains. Song, Jiangfeng,Liu, Chunquan,Li, Dajing,Song, Jiangfeng,Gu, Zhenxin.

[14]Effects of koji-making with mixed strains on physicochemical and sensory properties of Chinese-type soy sauce. Chen, Zhi-Yao,Feng, Yun-Zi,Cui, Chun,Zhao, Hai-Feng,Zhao, Mou-Ming,Chen, Zhi-Yao.

[15]Proteolysis and cathepsin activities in the processing of dry-cured duck. Wang, D. Y.,Xu, X. L.,Zhou, G. H.,Wang, P.,Wang, D. Y.,Zhang, M. H.,Bian, H.,Dong, H.,Xu, W. M.,Zhu, Y. Z.,Liu, F.,Geng, Z. M.. 2014

[16]Microbial Diversity and Biochemical Analysis of Suanzhou: A Traditional Chinese Fermented Cereal Gruel. Qin, Huibin,Sun, Qinghui,Pan, Xuewei,Yang, Hongjiang,Qin, Huibin,Qiao, Zhijun. 2016

[17]Integrated Transcriptome and Metabolic Analyses Reveals Novel Insights into Free Amino Acid Metabolism in Huangjinya Tea Cultiva. Zhang, Qunfeng,Liu, Meiya,Ruan, Jianyun,Zhang, Qunfeng,Liu, Meiya,Ruan, Jianyun. 2017

[18]Evaluation of sugar, free amino acid, and organic acid compositions of different varieties of vegetable soybean (Glycine max [L.] Merr). Song, Jiangfeng,Gu, Zhenxin,Song, Jiangfeng,Liu, Chunquan,Li, Dajing,Liu, Chunquan. 2013

[19]Simultaneous HPLC Determination of Amino Acids in Tea Infusion Coupled to Pre-column Derivatization with 2,4-Dinitrofluorobenzene. Li, Nana,Liu, Yang,Zhao, Yue,Zheng, Xinqiang,Lu, Jianliang,Liang, Yuerong,Li, Nana.

[20]The effect of dietary taurine supplementation on growth performance, feed utilization and taurine contents in tissues of juvenile white shrimp (Litopenaeus vannamei, Boone, 1931) fed with low-fishmeal diets. Yue, Yi-Rong,Liu, Yong-Jian,Tian, Li-Xia,Gan, Lian,Yang, Hui-Jun,Liang, Gui-Ying,He, Ju-Yun,Yue, Yi-Rong,Gan, Lian. 2013

作者其他论文 更多>>