Isolation and Characteristics of the CN Gene, a Tobacco Mosaic Virus Resistance N Gene Homolog, from Tobacco

文献类型： 外文期刊

第一作者： Zhang, Gai-Yun

作者： Zhang, Gai-Yun;Guo, Jia-Ming;Xu, Tong-Wen;Chen, Xue-Ping;Chen, Ming;Li, Lian-Cheng;Xu, Zhao-Shi;Ma, You-Zhi

作者机构：

关键词： Hypersensitive response;Systemic acquired resistance;TMV;Tobacco

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）

ISSN： 0006-2928

年卷期： 2009 年 47 卷 3-4 期

页码：

收录情况： SCI

摘要： Nicotiana rustica L. HZNH, a native Chinese tobacco germplasm, displays a hypersensitive response (HR) and systemic acquired resistance following infection with tobacco mosaic virus (TMV). A resistance gene, CN, cloned from HZNH plants, was homologous to the N and NH genes identified in other Nicotiana species. The CN coding region (3423 bp) shares 93.63% and 86.50% nucleotide identity with N and NH, respectively. Whereas the five CN exon sequences are highly homologous with those of N and NH, the four introns differ significantly in length and sequence. Sequence analysis revealed that CN belongs to the TIR/NBS/LRR gene class. Expression of CN was up-regulated after TMV infection and was temperature sensitive. Organ-specific expression analysis suggested that CN transcripts accumulated at high levels in leaves, low levels in stems, and minimal levels in roots. When CN was inserted into TMV-susceptible N. tabacum cv. K326 plants by Agrobacterium-mediated transformation, the transgenic plants displayed HR and systemic HR due to uninhibited movement of the virus.

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