Identification of the proteome composition occurring during the course of embryonic development of bees (Apis mellifera)
文献类型: 外文期刊
第一作者: Zhang, Z.
作者: Zhang, Z.;Pan, Y.;Li, J.;Zhang, L.;Feng, M.
作者机构:
关键词: honeybees (Apis mellifera L;);honeybee embryo;peptide mass fingerprint;proteome;MALDI-TOF
期刊名称:INSECT MOLECULAR BIOLOGY ( 影响因子:3.585; 五年影响因子:3.215 )
ISSN: 0962-1075
年卷期: 2009 年 18 卷 1 期
页码:
收录情况: SCI
摘要: To investigate the proteome during embryonic development of honeybees, Apis mellifera, proteins were identified by two-dimensional gel electrophoresis, mass spectrometry and protein engine identification tools that were applied to MASCOT and Xproteo search engines. 312, 320, 315 proteins were detected in 24, 48 and 72 h embryos. Thirty-eight highly abundant proteins were identified at the three time points by MS fingerprinting. All 21 proteins could be identified as products of annotated genes of the honeybee. Identified proteins included six proteins related to the metabolism of carbohydrates and energy production, six proteins belonging to the heat shock protein family, three cytoskeletal proteins, four proteins related to the antioxidant system of the embryo and two proteins related to growth regulation of the embryo. Quantitative proteomics was applied to analyze differences in amounts of these proteins during the three above mentioned developmental stages. Our data present an initial molecular picture of honeybee embryos, and will hopefully pave the way for future research on this animal.
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