Effect of N-terminal and C-terminal Deletion of Cry1Ie of Bacillus thuringiensis on its Expression and Purification

文献类型： 外文期刊

第一作者： Zhang, Chunlu

作者： Zhang, Chunlu;Guo, Shuyuan;Zhang, Jie;Song, Fuping

作者机构：

关键词： Bacillus thuringienesis;insecticidal protein;Cry1Ie;mutant

期刊名称：2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11

ISSN：

年卷期： 2009 年

页码：

收录情况： SCI

摘要： Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

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