Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences
文献类型: 外文期刊
第一作者: Niu, Qingli
作者: Niu, Qingli;Luo, Jianxun;Guan, Guiquan;Liu, Zhijie;Ma, Miling;Liu, Aihong;Gao, Jinliang;Ren, Qiaoyun;Li, Youquan;Yin, H.;Niu, Qingli;Qiu, Jiaxiang
作者机构:
关键词: Differentiation;Babesia sp.;Internal transcribed spacer;Ovine;Babesia moatsi
期刊名称:EXPERIMENTAL PARASITOLOGY ( 影响因子:2.011; 五年影响因子:2.132 )
ISSN: 0014-4894
年卷期: 2009 年 121 卷 1 期
页码:
收录情况: SCI
摘要: The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene for six Babesia spp. isolated from different geographic origins were characterized. Varying degrees of ITS1 and ITS2 intra- and inter-species sequence polymorphism were found among these isolates. Phylogenetic analysis of the ITS1-5.8S gene-ITS2 region clearly separated the isolates into two clusters. One held an unidentified Babesia sp. transmitted by Hyalomma anatolicum anatolicum. The second held five other isolates, which were considered to be Babesia motasi. Each Babesia species cluster possessed ITS1 and ITS2 of unique size(s) and species specific nucleoticle sequences. The results showed that ITS1, ITS2 and the complete ITS1-5.8S-ITS2 region could be used to discriminate these ovine Babesia spp. effectively. (C) 2008 Elsevier Inc. All rights reserved.
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