Purification, characterization. and gene cloning of an alkaline serine protease from a highly virulent strain of the nematode-endoparasitic fungus Hirsutella rhossiliensis

文献类型： 外文期刊

第一作者： Liu, Xingzhong

作者： Liu, Xingzhong;Wang, Bin;Liu, Xiaoying;Wu, Wenping;Li, Shidong

作者机构：

关键词： Hirsutella rhossiliensis;Nematode-endoparasitic fungus;Serine protease;Virulence factor

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

ISSN： 0944-5013

年卷期： 2009 年 164 卷 6 期

页码：

收录情况： SCI

摘要： Hirsutella rhossiliensis OWVT-1 has substantial potential as a biocontrol agent against plant-parasitic nematodes. Serine proteases have emerged as a potentially useful factor in the nematode-fungus interactions. When grown in liquid culture with the nematode Panagrellus redivivus as the sate nitrogen source, an extracellular alkaline protease (Hasp) was produced by the OWVT-1. The purified Hasp killed the juveniles of the soybean-cyst nematode (Heterodera glycines) and degraded proteins of the nematode cuticle. The molecular mass of Hasp was estimated to be 33 kDa. The optimum pH and temperature for enzyme activity were pH 9 and 75 degrees C. The amino acid sequence obtained by the N-terminal sequence analysis was applied for the primer design to isolate the Hasp cDNA gene, which consists of 1170bp open reading frame. Analysis of the cDNA and corresponding genomic sequence revealed that Hasp included four exons (279, 186, 513, and 192 bp) divided by three introns (65, 99, and 93 bp). Southern blotting showed that Hasp was a single-copy gene in the genome. The deduced amino acid sequence was very similar to other serine proteases of endoparasitic and egg-parasitic fungi of nematodes and of entomopathogenic fungi but was less similar to the serine proteases of nematode-trapping fungi. In a phylogenetic analysis of the amino acid sequences of serine proteases, the serine protease of H. rhossiliensis OWVT-1 clustered with the serine proteases of parasites of nematode eggs rather than with those of the trapping fungi. (C) 2009 Etsevier GmbH. All rights reserved.

分类号：

  • 相关文献

[1]Purification and characteristics of serine protease from the head of pacific white shrimp. Wu, Yanyan,Li, Laihao,Yang, Xianqing,Diao, Shiqiang,Wang, Ping. 2012

[2]A novel serine protease with clip domain from scallop Chlamys farreri. Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,Li, Chenghua,Xu, Wei,Zhu, Ling,Mao, Yuze. 2008

[3]Serine Proteases of Parasitic Helminths. Yang, Yong,Wen, Yun jun,Cheng, Shi Peng,Yang, Yong,Vallee, Isabelle,Boireau, Pascal,Cai, Ya Nan,Liu, Ming Yuan,Liu, Ming Yuan. 2015

[4]Characterization of roles of SpaA in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells. Zhu, Weifeng,Cai, Chengzhi,Wang, Ya,Li, Jingtao,Kang, Chao,Sun, Xiaomei,Jin, Meilin,Zhu, Weifeng,Cai, Chengzhi,Wang, Ya,Li, Jingtao,Kang, Chao,Sun, Xiaomei,Jin, Meilin,Wu, Chao,Sun, Xiaomei,Jin, Meilin,Wu, Chao,Sun, Xiaomei,Jin, Meilin,Zhu, Weifeng. 2017

[5]Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin. Dong, Jing,Ding, Hao,Liu, Yongtao,Yang, Qiuhong,Xu, Ning,Yang, Yibin,Ai, Xiaohui,Dong, Jing,Ding, Hao,Liu, Yongtao,Yang, Qiuhong,Xu, Ning,Yang, Yibin,Ai, Xiaohui. 2017

[6]Complete Genome Sequence of Aeromonas veronii Strain B565. Liu, Yuchun,Zhang, Yuting,Zhou, Zhigang,Li, Yanxia,Zhou, Zhemin,Huang, Huoqing,Ren, Yan,Li, Guannan,Wang, Lei,Zhou, Zhemin,Ren, Yan,Wang, Lei,Ren, Yan,Wang, Lei.

[7]Morin hydrate attenuates Staphylococcus aureus virulence by inhibiting the self-assembly of alpha-hemolysin. Wang, J.,Zhou, X.,Liu, S.,Li, G.,Shi, L.,Dong, J.,Li, W.,Deng, X.,Niu, X.,Dong, J..

[8]Genome-Wide Analysis of the First Sequenced Mycoplasma capricolum subsp capripneumoniae Strain M1601. Chen, Shengli,Hao, Huafang,Zhao, Ping,He, Ying,Gao, Pengcheng,Guo, Han,Ji, Wenheng,Wang, Zhanhui,Lu, Zhongxin,Chu, Yuefeng,Liu, Yongsheng,Thiaucourt, Francois,Thiaucourt, Francois. 2017

[9]Differential proteomics of outer membrane proteins between virulent and avirulent strains of Riemerella anatipestifer. Liu Yan,Wei Qiang,Bao Guo-Lian,Ji Quan-An. 2008

[10]Identification of lysophospholipase protein from Spiroplasma eriocheiris and verification of its function. Zhu, Huanxi,Liu, Peng,Du, Jie,Wang, Jian,Jing, Yunting,Zhang, Jia,Gu, Wei,Wang, Wen,Meng, Qingguo,Zhu, Huanxi,Liu, Peng,Du, Jie,Wang, Jian,Jing, Yunting,Zhang, Jia,Gu, Wei,Wang, Wen,Meng, Qingguo,Zhu, Huanxi,Gu, Wei,Meng, Qingguo.

作者其他论文 更多>>

微信小程序