Establishment and characteristics of white ear lobe chicken embryo fibroblast line and expression of six fluorescent proteins in the cells

文献类型： 外文期刊

第一作者： Wu, Hongmei

作者： Wu, Hongmei;Guan, Weijun;Li, Han;Ma, Yuehui

作者机构：

关键词： White ear lobe chicken embryo;Fibroblast bank;Biological characteristics

期刊名称：CELL BIOLOGY INTERNATIONAL （ 影响因子：3.612； 五年影响因子：3.055 ）

ISSN： 1065-6995

年卷期： 2008 年 32 卷 12 期

页码：

收录情况： SCI

摘要： A white ear lobe chicken embryo (WELCE) fibroblast cell bank, containing 322 tubes of frozen cells, was successfully established from primary explants of 57 embryo samples. The cells were morphologically consistent with fibroblasts, and the growth curve was sigmoidal with a population doubling time (PDT) of 48 h. Karyotyping and G-banding indicated a total chromosome number of 2n 78; the rate of diploidy in the cell bank was 97.62%. The cells were also free from bacterial, fungal, viral and mycoplasma contamination. Analysis of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isoenzymes ruled out cross-contamination between cells. In order to study exogenous gene expression, six fluorescent proteins were transfected into the WELCE cells. The transfection efficiency of these genes was between 10.1 and 41.9%. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 24 h after transfection. The results indicate that the quality of the cell line meet the quality requirements of the ATCC (American Type Culture Collection). (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

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