Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus

文献类型: 外文期刊

第一作者: Chen, Hao-tai

作者: Chen, Hao-tai;Zhang, Jie;Sun, De-hui;Ma, Li-na;Liu, Xiang-tao;Liu, Yong-sheng;Quan, Kai

作者机构:

关键词: Porcine reproductive and respiratory;syndrome virus (PRRSV);Highly pathogenic;Detection;Reverse transcription loop-mediated;isothermal amplification (RT-LAMP)

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN: 0166-0934

年卷期: 2008 年 153 卷 2 期

页码:

收录情况: SCI

摘要: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the open reading frames la of highly pathogenic porcine reproductive and respiratory syndrome virus genome was developed. The 10 reference strains, I clinical isolation strain and 122 positive samples were tested. Positive reactions were confirmed for all strains and specimens by reverse transcription loop-mediated isothermal amplification and nested reverse transcription polymerase chain reaction (RT-PCR). The results showed this detection technique is more reliable and convenient for rapid and sensitive diagnosis of highly pathogenic porcine reproductive and respiratory syndrome virus infection. (C) 2008 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]Multiplex reverse transcription loop-mediated isothermal amplification for the simultaneous detection of CVB and CSVd in chrysanthemum. Muhammad, Imtiaz,Hong, Bo,Liu, Xing-liang,Zhao, Xi-ting,Ge, Bei-bei.

[2]Development and evaluation of reverse transcription loop-mediated isothermal amplification assay for the detection of the fathead minnow nidovirus. Zhang, Qingli,Zhang, Qingli,Standish, Isaac,Winters, Andrew D.,Faisal, Mohamed,Winters, Andrew D.,Faisal, Mohamed,Puzach, Corey,Ulferts, Rachel,Ziebuhr, John,Ziebuhr, John. 2014

[3]Identification of dispensable nucleotide sequence in 3 ' untranslated region of porcine reproductive and respiratory syndrome virus. Sun, Zhi,Liu, Changlong,Tan, Feifei,Gao, Fei,Yuan, Shishan,Sun, Zhi,Qin, Aijian,Liu, Ping. 2010

[4]Chimeric porcine reproductive and respiratory syndrome viruses reveal full function of genotype 1 envelope proteins in the backbone of genotype 2. Tian, Debin,Zheng, Haihong,Zhang, Rong,Zhuang, Jinshan,Yuan, Shishan. 2011

[5]Syndecan-4, a PRRSV attachment factor, mediates PRRSV entry through its interaction with EGFR. Wang, Rui,Wang, Xin,Ni, Bo,Huan, Chang-chao,Fan, Hong-jie,Mao, Xiang,Liao, Ying,Tong, Guang-zhi,Ding, Chan,Mao, Xiang,Wu, Jia-qiang,Wen, Li-bin.

[6]Efficient porcine reproductive and respiratory syndrome virus entry in MARC-145 cells requires EGFR-PI3K-AKT-LIMK1-COFILIN signaling pathway. Wang, Rui,Wang, Xin,Ni, Bo,Huang, Li,Mao, Xiang,Liao, Ying,Tong, Guang-zhi,Ding, Chan,Mao, Xiang,Wu, Jia-qiang,Wen, Li-bing.

[7]A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. Kang, Kang,Luo, Jun,Kang, Kang,Zhong, Jiasheng,Zhang, Limin,Gou, Deming,Yang, Keli,Tian, Yongxiang,Zhai, Jianxin,Zhang, Li,Song, Changxu,Gou, Christine Yuan. 2014

[8]Label-Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain. Qu, Zehui,Gao, Fei,Li, Liwei,Zhang, Yujiao,Jiang, Yifeng,Yu, Lingxue,Zhou, Yanjun,Zheng, Hao,Tong, Wu,Li, Guoxin,Tong, Guangzhi,Qu, Zehui,Jiang, Yifeng,Yu, Lingxue,Zhou, Yanjun,Zheng, Hao,Tong, Wu,Li, Guoxin,Tong, Guangzhi. 2017

[9]Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome in Jiangxi province, China. Guo, Aijiang,Wu, Guohua,Gong, Wei,Luo, Xuenong,Zheng, Haixue,Jia, Huanjie,Cai, Xuepeng. 2012

[10]H7N9 virulent mutants detected in chickens in China pose an increased threat to humans. Shi, Jianzhong,Deng, Guohua,Kong, Huihui,Gu, Chunyang,Ma, Shujie,Yin, Xin,Zeng, Xianying,Cui, Pengfei,Chen, Yan,Yang, Huanliang,Wan, Xiaopeng,Wang, Xiurong,Liu, Liling,Chen, Pucheng,Jiang, Yongping,Liu, Jinxiong,Guan, Yuntao,Li, Mei,Qu, Zhiyuan,Guan, Lizheng,Zang, Jinkai,Gu, Wenli,Han, Shuyu,Song, Yangming,Hu, Yuzhen,Wang, Zeng,Gu, Linlin,Yang, Wenyu,Liang, Libin,Bao, Hongmei,Tian, Guobin,Li, Yanbing,Qiao, Chuanling,Jiang, Li,Li, Chengjun,Bu, Zhigao,Chen, Hualan,Suzuki, Yasuo. 2017

[11]A Multiplex RT-PCR Assay to Detect and Discriminate Porcine Reproductive and Respiratory Syndrome Viruses in Clinical Specimens. Yang, Keli,Tian, Yongxiang,Zhou, Danna,Duan, Zhengying,Guo, Rui,Liu, Zewen,Yuan, Fangyan,Liu, Wei. 2017

[12]Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China. Fan, Baochao,Yu, Zhengyu,Pang, Fengjiao,Xu, Xiangwei,Zhang, Baimeng,Guo, Rongli,He, Kongwang,Li, Bin,Fan, Baochao,Yu, Zhengyu,Pang, Fengjiao,Xu, Xiangwei,Zhang, Baimeng,Guo, Rongli,He, Kongwang,Li, Bin.

[13]A one-step RT-PCR assay to detect and discriminate porcine reproductive and respiratory syndrome viruses in clinical specimens. Yang, Keli,Duan, Zhengying,Guo, Rui,Liu, Zewen,Zhou, Danna,Yuan, Fangyan,Tian, Yongxiang,Li, Yanhe.

[14]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[15]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[16]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[17]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[18]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[19]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[20]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

作者其他论文 更多>>