Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus
文献类型: 外文期刊
第一作者: Chen, Hao-tai
作者: Chen, Hao-tai;Zhang, Jie;Sun, De-hui;Ma, Li-na;Liu, Xiang-tao;Liu, Yong-sheng;Quan, Kai
作者机构:
关键词: Porcine reproductive and respiratory;syndrome virus (PRRSV);Highly pathogenic;Detection;Reverse transcription loop-mediated;isothermal amplification (RT-LAMP)
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN: 0166-0934
年卷期: 2008 年 153 卷 2 期
页码:
收录情况: SCI
摘要: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the open reading frames la of highly pathogenic porcine reproductive and respiratory syndrome virus genome was developed. The 10 reference strains, I clinical isolation strain and 122 positive samples were tested. Positive reactions were confirmed for all strains and specimens by reverse transcription loop-mediated isothermal amplification and nested reverse transcription polymerase chain reaction (RT-PCR). The results showed this detection technique is more reliable and convenient for rapid and sensitive diagnosis of highly pathogenic porcine reproductive and respiratory syndrome virus infection. (C) 2008 Elsevier B.V. All rights reserved.
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