A chromatographic strip test for rapid detection of one lineage of the H5 subtype of highly pathogenic avian influenza

文献类型： 外文期刊

第一作者： Cui, Shangjin

作者： Cui, Shangjin;Tong, Guangzhi

作者机构：

关键词： Avian influenza virus;colloid gold test strip;H5 subtype;monoclonal antibody

期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）

ISSN： 1040-6387

年卷期： 2008 年 20 卷 5 期

页码：

收录情况： SCI

摘要： A rapid immune colloidal gold test strip for detecting I lineage of the H5 subtype of highly pathogenic avian influenza was developed based on membrane chromatography with monoclonal antibodies (mAbs) against hemagglutinin (HA) of the H5 subtype of Avian influenza Virus (AIV). Bagg albino C mice that were immunized with AIV A/Goose/Guangdong/3/96(H5N1) yielded 4 hybridomas secreting mAbs (1A6, 1B8, 3C4, and 8F6) specific for HA. Monoclonal antibody 3C4 was conjugated with colloidal gold as the detector antibody. On the test strip, 1A6, in combination with rabbit anti-H5N1 polyclonal antibody, was used as the capture complex at the "test line," and goat antimouse immunoglobulin G antibody was used as the capture antibody at the "control line." The strip test correctly detected the H5 Subtype of AIV in known positive samples and did not react with the standard antigens of AIV H1-H4 or H6-H14, or the antigens of Newcastle disease virus, Infectious bursal disease virus, Infections bronchitis virus, or Avian infectious laryngotracheitis virus. When 483 clinical specimens Suspected of infection with H5N1 were tested, results from the strip test were nearly identical with those from the HA/hemagglutination inhibition test, reverse transcription polymerase chain reaction, and a commercial enzyme-linked immunosorbent assay kit. Because the newly developed strip was specific, rapid, and sensitive, it should be useful for diagnosing the H5 Subtype of AIV and for investigating its epidemiology. The test strip is only reliable for the lineage of H5N1 As for the other strains of AIV, further experiments will be needed.

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